rodent and rabbit assay data sheet
S0231 - Ultrasensitive qualitative detection of rabbit
coronavirus by reverse transcription coupled real time PCR
Coronaviruses (CoVs) are found in a wide variety of animals, in which
they can cause respiratory, enteric, hepatic, and neurological
diseases of various severities. Recent taxonomy revision has
placed coronaviruses into three genera, alphacoronavirus,
betacoronavirus, and gammacoronavirus, replacing the traditional
group 1, 2, and 3 CoVs. Recently, novel CoVs have been
discovered in birds and pigs, giving rise to a new genus,
Due to their unique mechanism of viral replication, CoVs have a high
frequency of recombination and because of their high mutation
rates as result of RNA replication, they can adapt to new hosts
and ecological niches easily.
Rabbit CoVs belong to betacoronavirus subgroup A. Rabbit CoVs have been
observed in feces of clinically healthy adult rabbits and these
sub-clinically infected rabbits can shed the virus for a long
time. The virus is usually transmitted by fecal-oral route. This
viral infection has been reported in some cases to cause acute
intestinal disease with diarrhea and high mortality in young
rabbits. However, more commonly it causes only mild diarrhea and
is often only clinically significant with concurrent
E. coli infection.
Culture detection of this virus is usually very slow and not sensitive.
Immunological detection is hampered by the fact that prior
exposure to other coronaviruses can result in cross-reactivity.
Molecular detection by polymerase chain reaction (PCR) is fast,
sensitive and specific (Van Elden et al., 2004).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of rabbit coronavirus
Help ensure that rabbit colonies or populations are free of
Early prevention of spread of this virus among a
population or in a geographic area
Minimize human exposure to this virus
Safety monitoring of biological products that derive
Van Elden, L.J.R. et al (2004) Frequent detection of human coronaviruses
in clinical specimens from patients with respiratory tract
infection by use of a novel real-time reverse-transcriptase
polymerase chain reaction. J. Infect. Dis., 189: 652–657.
0.2 ml feces, or fecal or rectal swab, or 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for more information.
2 business days
reverse transcription coupled real time polymerase chain reaction