Mouse kidney parvovirus

Test code: S0246 - Ultrasensitive qualitative detection of mouse kidney parvovirus (MKV or MKPV) by real time polymerase chain reaction

Mouse kidney parvovirus (MKV or MKPV) is a recently discovered parvovirus in mouse. It is also known as murine chapparvovirus (MuCPV). The genetic sequence of this new strain of parvovirus is quite different from that of more familiar mouse parvoviruses, such as mouse parvovirus-1 and mouse minute virus. MKPV is a single-stranded DNA parvovirus, only distantly related to other known mouse parvoviruses; it is more similar to other members of the provisional genus Chapparvovirus.

In immunodeficient mice, infection with MKV can result in severe chronic interstitial nephropathy and renal failure within a few months. For healthy mice, MKV infection can have a variable disease course. Some infected mice may be asymptomatic and continue shedding the virus through their feces and urine. Multiple animal facilities around the world have reported infection of their mice with this virus (Ge, Z. et al., 2020). Co-housing with inapparent carrier MKV-infected mice is the major cause of the spread of this virus.

Identification of this virus mainly relies on detecting the viral genome using PCR, which is rapid and specific (Ge, Z. et al., 2020).

Utilities:

• Help confirm the disease causing agent
• Shorten the time required to confirm a clinical diagnosis of MKPV infection
• Help ensure that rodent colonies are free of this virus
• Early prevention of spread of MKPV among a colony
• Minimize human exposure to this virus
• Safety monitoring of biological products that derive from rodents

References:
Ge, Z., Carrasco, S.E., Feng, Y., Bakthavatchalu, V., Annamalai, D., Kramer, R., Muthupalani, S. and Fox, J.G. (2020) Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice. Emerg. Microbes Infect. 9:1814-1823.

Specimen requirements: Fecal pellet, or 0.2 ml EDTA whole blood, or 0.2 ml serum or plasma or urine.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected