Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum





Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)




Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Dengue virus PCR test

equine assay data sheet


Test code:
S0060 - Ultrasensitive qualitative detection of Dengue virus by reverse transcription coupled real time polymerase chain reaction. Assay detects but does not differentiate common strains of Dengue virus types 1, 2, 3 and 4.


Dengue viruses belong to the family Flaviviridae which contains close to 70 different viruses. These include viruses causing yellow fever and several encephalitides, such as Japanese encephalitis and tick-borne encephalitis. There are four distinct serotypes of Dengue virus that are all mosquito-borne (Gubler, 1998; Halstead, 1997). Each of the four Dengue virus serotypes can produce a wide spectrum of disease severity. The Dengue fever (DF) is the most common, and occurs more frequently among older children and adults. DF is self-limiting and is characterized by a sudden onset of nonspecific indicators such as headache, myalgia, arthralgia and rashes. DF can range in severity from a minor infirmity to a temporarily incapacitating syndrome with residual fatigue and depression.

At the extreme of Dengue virus infection is the Dengue hemorrhagic fever (DHF), which primarily affects children under the age of 10 years. The clinical symptoms of DHF include plasma leakage, a bleeding tendency, and liver involvement, which may lead to potentially life-threatening syndromes including disseminated intravascular coagulation and Dengue shock syndrome. There are no vaccines for Dengue, and treatment is limited to supportive therapies. It is estimated that 50-100 million people suffer from Dengue fever annually and hundreds of thousands of cases of Dengue hemorrhagic fever occur in the tropics every year (Gubler, 1998; Halstead, 1997).

The virus contains a positive RNA genomic strand approximately 11 kb in length. Studies of evolutionary relationships between the four serologically distinct Dengue viruses have shown that each serotype can further be subdivided into several major genotypes or monophyletic groups. For example, five genotypes or monophyletic groups have been described for Dengue 1 virus.

PCR detection of Dengue virus is not only quick (usually less than 2 days) but is also sensitive and specific.


  • Help confirm the disease causing agent
  • Help ensure that herds are free of Dengue virus
  • Early prevention of spread of the virus among a herd
  • Minimize personnel exposure to the virus
  • Safety monitoring of biological products and vaccines that derive from horses

Furumizo, R.T. and Rudnick, A. (1979) Laboratory observations on the life history of two species of the Aedes (Finlaya) niveus subgroup (Diptera: Culicidae) in Malaysia. J Med Entomol 15: 573-575.
Gubler, D.J. (1998) Dengue and dengue hemorrhagic fever. Clin. Microbiol. Rev. 11: 480-496.
Halstead, S.B. (1997) Epidemiology of dengue and dengue hemorrhagic fever, p. 23-44. In D. J. Gubler and G. Kuno (ed.) Dengue and dengue hemorrhagic fever. CAB International, Wallingford, United Kingdom.
Scherer, W.F., Russell, P.K., Rosen, L., Casals, J. and Dickerman RW (1978) Experimental infection of chimpanzees with dengue viruses. Am J Trop Med Hyg 27: 594-599.
Wolfe, N.D., Kilbourn, A.M., Karesh, W.B., Rahman, H.A., Bosi, E.J., Cropp, B.C., Andau, M., Spielman, A. and Gubler, D.J. (2001) Sylvatic transmission of arboviruses among Bornean orangutans. Am. J. Trop. Med. Hyg. 64:310-6.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml plasma or serum.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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