Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum





Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)




Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Strongyles PCR test for horses
equine assay data sheet

Strongyles worms in horses

Test code:
X0016 - Ultrasensitive qualitative detection but not differentiation of most strongyles species in horses, by real time PCR.


Nematodes of the subfamily Cyathostominae (cyathostomins), order Strongylida, are the most common parasites of horses. More than 50 species within 13 genera have been documented, including Cylicocyclus ashworthi, Cylicocyclus auriculatus, Cylicocyclus radiatus, Cylicocyclus insigne, Cylicocyclus leptostomum, Cylicocyclus nassatus, Cylicostephanus bidentatus, Cylicostephanus calicatus, Cylicostephanus goldi, Cylicostephanus longibursatus, Cylicostephanus minutus, Cyathostomum pateratum, Cyathostomum catinatum, Coronocyclus coronatus, Coronocyclus labiatus and Tridentoinfundibulum gobi. Infected horses often carry five to ten common species.

Horses infected with these parasites may develop various syndromes including larval cyathostominosis, a fatal enteritis that occurs secondary to synchronized reactivation of inhibited larvae from the intestinal mucosa. The mortality rate is 50% even when intensive treatment is given. Other syndromes induced by these nematodes include a protein-losing enteropathy with loss of condition and peripheral edema, or mild weight loss, poor appetite and lethargy with disordered intestinal motility. The association between the individual strongyles species and the development of these different clinical syndromes is not yet well understood.

Horses that have been exposed to these parasites do not always develop immunity to the parasites. Infected horses can contaminate pastures throughout their lives.

The current method of detecting strongyles infections is to remove the adult worms at necropsy and examine their morphology, which is of little value in clinical veterinary practice. Detecting eggs or larvae in stool samples is difficult and time-consuming using morphological parameters. However, molecular detection by PCR is a highly sensitive and specific technique for detecting these parasites.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm the presence of strongyles
  • Help ensure that herds are free of strongyles
  • Early prevention of spread of these parasites among a herd or to other animals
  • Minimize human exposure to strongyles

Specimen requirements: 0.2 ml feces or rectal swab.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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