Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum





Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)




Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Helicobacter PCR tests for horses
equine assay data sheet

Helicobacter species

Test codes:

B0021 - Ultrasensitive qualitative detection of Helicobacter pylori by real time polymerase chain reaction.

B0023 - Ultrasensitive qualitative detection of Helicobacter heilmannii by real time polymerase chain reaction.

P0010 - Ultrasensitive Helicobacter species screen by real time polymerase chain reaction.  This test detects but does not differentiate H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.

P0011 - Ultrasensitive Helicobacter species identification by real time polymerase chain reaction and PCR product sequence analysis.  This test detects and differentiates H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.


Many species of the genus Helicobacter have been identified in mammals and their pathogenicity varies. Some species can induce significant disease while others appear to merely colonize the gastrointestinal tract.

Helicobacter pylori is a gram-negative spiral bacterium found in gastric mucosa and associated with active and chronic gastritis. H. pylori can establish a chronic, persistent infection, which may lead to gastric or duodenual ulcers, gastric cancer and gastric lymphomas. Studies have revealed that approximately 50% of the world’s human population is infected with H. pylori.

Biochemically, the bacterium produces catalase, oxidase and urease enzymes. The urease enzyme permits the bacterium to metabolize urea present in the gastric mucosa and establish a microenvironment favorable to the organism. H. pylori is a highly motile organism with multiple unipolar flagella. Both the urease enzyme and the flagella are considered to be important virulence factors.

Diagnosis of Helicobacter pylori infection in humans relies on upper endoscopy or the 13C-urea breath test (see review by Nakamura, 2001). Although the endoscopy procedure permits culture of the bacterium from biopsy specimens (the gold standard for diagnosis), demonstration of urease activity and histological detection of the germ, the procedure is expensive and invasive. The 13C-urea breath test is a well-established, relatively sensitive, specific and noninvasive method. Molecular tests, such as PCR, can also offer precise diagnosis of H. pylori infections. In fact, molecular testing by PCR can complement other diagnostic tests because it can be applied to archival fixed tissue, environmental samples, gastric fluid, oral secretions, and stool samples, in which traditional diagnostic tests do not have sensitivity and perform poorly. Studies have shown than PCR detection of H. pylori in gastric fluid specimens can reach a sensitivity of 96% and a specificity of 100% (Westblom et al., 1993; Yoshida et al., 1999). This capability is especially useful in monitoring active H. pylori infection in rodents and other animals, as the breath test is difficult to conduct for these animals.

Helicobacter heilmannii (previously known as Gastrospirillum hominis) is a 4-10 m long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 m, up to 14 uni- or bipolar flagella, and no periplasmic filaments. In humans, gastric infection with H. heilmannii is associated with the development of chronic gastritis (found in the stomachs of 0.2 to 4% of patients with gastritis) and low-grade mucosa-associated lymphoid tissue lymphoma in humans. Eradication of H. heilmannii by antibiotic treatment of patients can result in complete remission of MALT lymphoma, indicating a causal relationship between H. heilmannii infection and MALT lymphoma. Unlike H. pylori infections, gastric infections with H. heilmannii or Gastrospirillum-like organisms are not restricted to humans. A broad range of animals including dogs, cats, pigs, and cattle are naturally infected, with frequencies ranging from 80% to 100%. It has been suggested that H. heilmannii infection in humans may be a zoonosis and that animals may serve as a reservoir for transmission to humans.

Definitive culture of H. heilmannii has not been achieved to date (Anderson et al., 1996) and diagnosis of H. heilmannii infection is usually made on the basis of its distinct spiral morphology, compared with H. pylori, on silver- stained tissue sections. However, there are a number of large, gastric, spiral organisms such as H. felis, H. salomonis, and H. bizzozeronii which are indistinguishable from H. heilmannii on routine light microscopy, and H. pylori grown in a broth culture can also adopt a morphology identical to that of H. heilmannii (Fawcett et al., 1999). Molecular detection methods such as PCR are required for more definitive identification (Trebesius et al., 2001).


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Helicobacter infection.
  • Help ensure that horse populations are free of Helicobacter species
  • Early prevention of spread of these bacteria
  • Minimize personnel exposure to these bacteria
  • Safety monitoring of biological products that derive from horses

Specimen requirements:  0.2 ml gastric lavage, or rectal swab, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days (1 week for P0011)

B0021, B0023, P0010
- Qualitative real time PCR
P0011 - Qualitative real time PCR + PCR product sequence analysis

Normal range: Nondetected

2003-2023 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880