Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum





Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)



Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Trypanosoma evansi PCR test for horses
equine assay data sheet

Surra (trypanosomiasis)
Etiologic agent: Trypanosoma evansi

Test code:
X0008 - Ultrasensitive qualitative detection of Trypanosoma evansi by real time polymerase chain reaction


Surra is an animal disease occurring in Africa, Asia and Latin America, caused by Trypanosoma evansi. T. evansi belongs to the subgenus Trypanozoon, together with T. equiperdum and T. brucei. The parasite infects various host species and is transmitted by biting flies such as Tabanidae and Stomoxys species, as well as by vampire bats including Desmodus rotondus (Hoare, 1972). Camels and horses are very susceptible to the infection and death can occur within weeks or months. T. evansi infections of cattle and buffalo usually lead to pronounced immunosuppression, resulting in increased susceptibility to other opportunistic diseases such as Pasteurella and anthrax (Stephen, 1986).

Diagnosis of T. evansi infection usually starts with clinical symptoms or the detection of antibodies to T. evansi. Conclusive evidence of T. evansi infection, however, relies on detection of the parasite in the blood or tissue fluids of infected animals. Unfortunately, parasitological techniques cannot always detect ongoing infections as the level of parasitemia is often low and fluctuating, particularly during the chronic stage of the disease (Nantulya, 1990).

Molecular detection of T. evansi using the polymerase chain reaction is a highly sensitive and specific alternative to parasitological tests.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Trypanosoma evansi infection.
  • Help ensure that horse populations are free of T. evansi
  • Early prevention of spread of this parasite
  • Minimize personnel exposure to this parasite
  • Safety monitoring of biological products that derive from horses

Hoare, C.A. (1972) The trypanosomes of mammals. Oxford, Blackwell Scientific Publications 1972, 1-749.
Nantulya, V.M. (1990) Trypanosomiasis in domestic animals: the problems of diagnosis.
Rev Sci Tech 1990, 9:357-367.
Stephen, L.E. (1986) Trypanosomiasis. A veterinary perspective (Edited by: StephenLE). Oxford, Pergamon Press 1986.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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