Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum





Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)




Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Japanese encephalitis PCR test for horses
equine assay data sheet


Test code:
S0062 - Ultrasensitive qualitative detection of Japanese encephalitis virus by reverse transcription coupled real time polymerase chain reaction


One of the leading causes of acute encephalopathy in children in the tropics is Japanese encephalitis (JE). An arbovirus, the Japanese encephalitis virus is transmitted by Culex mosquitoes and is a member of genus Flavivirus of the family Flaviviridae. The RNA genome of the JE virus is positive sense, single-stranded, approximately 11 Kb in length, and contains one long open reading frame.

JE virus is related to Murray Valley encephalitis virus. The virus is neurotropic and predominately affects the thalamus, anterior horns of the spinal cord, cerebral cortex, and cerebellum. It mainly affects children <15 years of age and is mostly asymptomatic. The occasional symptomatic child typically presents with a neurological syndrome characterized by altered sensorium, seizures, and features of intracranial hypertension. Though no antiviral drug is available against JE, effective supportive management can improve the outcome. Control of JE involves efficient vector control and appropriate use of vaccines.

Horses and primates can develop similar pathological lesions to those in human when infected with JE virus; they are considered dead-end hosts for JE. Most horses infected by JE virus show mild clinical signs, including fever, anorexia and depression. However, the mortality rate is high when JE infected horses show neurological symptoms (Ihara et al., 1997). Seroepidemiological survey of Asian monkeys has also shown widespread infection of these primates with JE virus (Yuwono et al., 1984). The virus can also infect birds, pigs and donkeys. Currently, the virus is mainly detected in East Asia, southeast Russia, India, Papua New Guinea and the Torres Strait Islands.

Conventional methods of JE diagnosis, including hemagglutination-inhibition and complement fixation tests for antibody assay, have been ineffective because of low sensitivity. Although a new immunoassay was developed to detect earlier, virus-specific IgM antibodies in the serum and cerebrospinal fluid (CSF) of acute and convalescent-phase patients, this new assay also suffered from low sensitivity and non-specific reaction. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to detect Flavivirus rapidly and specifically.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of JE infection.
  • Help ensure that animal populations are free of JE virus
  • Early prevention of spread of the virus
  • Minimize personnel exposure to the virus
  • Safety monitoring of biological products and vaccines that derive from horses and primates

Ihara, T., Kano, R., Nakajima, Y., Sugiura, T., Imagawa, H., Izuchi, T. and Samjima, T. (1997) Detection of antibody to Japanese encephalitis virus (JEV) by enzyme-linked immunosorbent assay (ELISA). J. Equine Sci. 8: 25-28.
Yuwono, J., Suharyono, W., Koiman, I., Tsuchiya, Y. and Tagaya, I. (1984) Seroepidemiological survey on dengue and Japanese encephalitis virus infections in Asian monkeys. Southeast Asian J Trop Med Public Health. 15:194-20

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh or frozen CNS tissue, or 0.2 ml CSF, serum or plasma.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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