Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Anoplocephala

Anoplocephaloides

Aspergillus

Babesia

Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dengue

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Equine rhinitis virus A

Equine rhinitis virus B

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Equine hepatitis PCR test

equine assay data sheet

Equine hepatitis virus (EqHV)

Test code: S0233 - Ultrasensitive qualitative detection of equine hepatitis virus (EqHV) by reverse transcription real time PCR.

 

Equine hepatitis virus (EqHV) or equine hepacivirus is a non-primate hepacivirus (NPHV), phylogenetically related to the human hepatitis C virus. EqHV is a single-stranded RNA virus containing an approximate 9.2 kb genome.

Horses have been infected with this virus in the United States, Japan, New Zealand, and Europe. EqHV can cause subclinical disease in adult horses with transient mild elevations in liver-derived serum enzymes. Similar to EqPV-H, EqHV can be found in healthy carriers with no clinical signs, which may serve as reservoirs for infection of other horses.

Transmission of EqHV is usually through veterinary products, such as vaccines, immune sera, or other equinederived blood products. In an attempt to eliminate the risk of equine serum hepatitis secondary to equine biologic product administration, the USDA Center for Veterinary Biologics has proposed that all licensed equine blood products be tested for EqPV-H and EqHV.

Serological detection of the virus is not very sensitive and is mainly used to identify horses who may have had prior exposure to the virus. Detection by viral culture is also not very sensitive, and is slow. Effective detection of active carriers of the virus relies on the use of molecular detection by polymerase chain reaction, which is rapid and specific (Tanaka et al., 2014).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of the presence of these viruses
  • Help ensure that horse populations are free of these viruses
  • Early prevention of spread of these viruses
  • Minimize human exposure to these viruses
  • Safety monitoring of biological products that derive from horses

References:
Tanaka, T., Kasai, H., Yamashita, A., Okuyama-Dobashi, K., Yasumoto, J., Maekawa, S., Enomoto, N., Okamoto, T., Matsuura, Y., Morimatsu, M., Manabe, N., Ochiai, K., Yamashita, K. and Moriishi, K. (2014) Hallmarks of hepatitis C virus in equine hepacivirus. J. Virol. 88:13352-66.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube; or 0.2 ml serum; or 0.2 ml fresh or frozen tissue; or 0.2 ml culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription real time PCR

Normal range: Nondetected

Equine hepatitis PCR test

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