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rodent and rabbit assay data sheet
Rat parvovirus (includes
Kilham rat virus, RPV/rat orphan parvovirus, and
H-1/Toolan’s)
Test code:
S0098 - Ultrasensitive qualitative detection
but not differentiation of Kilham rat virus and other common rat parvovirus
strains by real time PCR.
Test S0098 in included on the
P0063 Rat breeding PCR panel
Rat
parvovirus (including Kilham virus) is among the most prevalent infectious agents
found in rat colonies. There is significant potential for
rat parvovirus to be transmitted among animals within a breeding
colony due to
the high degree of environmental stability of this virus.
Rats are infected primarily by direct contact with virus shed in
feces or urine. Parvoviruses are capable of surviving in the
environment for an extended period of time (weeks). Thus fomite
transmission is likely.
Serology has been used in the past to test for this virus in
rats. However, this testing
approach is often inadequate to control the spread of this
virus, because it takes close to 2 weeks to generate a
detectable serological response after rats are exposed. Also, the sensitivity of serological testing
is often not as high as molecular detection by PCR. Fecal pellet samples can be
used to test for rat parvovirus by PCR, making sample collection
less invasive than serology testing.
Utilities:
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Help confirm the disease causing agent
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Shorten the time required to confirm a clinical
diagnosis of Kilham virus/rat parvoviruses
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Help ensure that rat breeding colonies are free of these viruses
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Early prevention of spread of these viruses among a
colony
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Minimize human exposure to these viruses
Specimen requirements:
Fecal pellet, or 0.2 ml urine, or 0.2 ml whole blood in EDTA
(purple top) tube, or 0.2 ml fresh, frozen
or fixed tissue,
or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
real time polymerase chain reaction
Normal range: Nondetected
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