Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum



Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)



Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)





Horsepox virus


Japanese encephalitis

Lawsonia intracellularis


Lyme disease


Neospora caninum

Neospora hughesi


Potomac horse fever


Rhodococcus equi


Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae




Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum


Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis

Leptospira PCR test for horses
equine assay data sheet


Test code:
B0051 - Ultrasensitive qualitative detection of Leptospira bacteria by real time polymerase chain reaction. This test detects but does not differentiate the Leptospira serovars that are typically of clinical concern in horses.

B0051 is included in P0024 - equine breeding panel


Leptospirosis is a disease of worldwide significance that infects many domestic and wildlife animal species and humans. The scientific name of the infecting organism is Leptospira interrogans sensu lato. Within this species there are many different strains (serovars).

Leptospira can be spread between animals through contact with infected urine, venereal or placental transfer, or bite wounds. Indirect transmission occurs through exposure of susceptible animals to contaminated water sources, food, bedding or human hands. Leptospira bacteria can survive in manure up to 2 months. Stagnant or slow moving water provides a suitable habitat; the organism can survive up to 20 days in water. This explains why outbreaks often increase during periods of flooding.

Fever, shivering and muscle tenderness are among the first signs of acute infection. Rapid dehydration may develop subsequently. In subacute infections, the animal usually develops a fever, anorexia, dehydration, and increased thirst. Animals with liver involvement may develop icterus. Conjunctivitis and recurrent uveitis may occur in chronic infections. Breeding success can be impacted because of increased risk of abortion and decreased milk production in mares.

There is currently no approved equine vaccine for leptospirosis. Although Leptospira infection can be diagnosed by change in antibody titers, titers may be negative during early stages of critical infection. Thus an alternative method is needed to provide a quick diagnosis of the disease at this stage. Molecular detection of PCR is rapid, highly sensitive and specific. It is suitable for quick diagnosis even during the first few days of infection.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Leptospira infection.
  • Help ensure that horse populations are free of Leptospira
  • Early prevention of spread of this bacterium
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products that derive from horses

Specimen requirements: Urogenital swab, cervical swab, or 0.2 ml urine, or 0.2 ml semen, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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