Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Strep equi and zoo PCR tests
equine assay data sheet

Streptococcus equi, subspecies equi ("strangles") and zooepidemicus ("strep zoo")

Test code:
B0019 - Ultrasensitive qualitative detection and differentiation of Streptococcus equi, subspecies equi and zooepidemicus by real time polymerase chain reaction.

B0019 is included in P0013 - equine respiratory panel and in P0024 - equine breeding panel.

 

Streptococcus equi subsp. equi is the etiologic agent of strangles and is responsible for nearly 30% of all reported equine infections worldwide (Chanter, 1997). Strangles is characterized by pharyngeal constriction in the horse's upper respiratory tract as a consequence of lymph node swelling and is often accompanied by abscessation. The very closely related organism Streptococcus zooepidemicus (S. equi subsp. zooepidemicus) is a significant cause of equine lower airway disease, foal pneumonia, endometritis, and abortion (Chanter, 1997). There is currently no effective vaccine or treatment for strangles.

In the past, identification of S. equi bacteria usually relied on culture of the bacteria, but this technique is slow and not very sensitive. A recent study (Newton, 2000) has shown that repeated nasopharyngeal swabbing and culture of Streptococcus equi could not detect the development of healthy carriers in more than 50% of strangles outbreaks. S. equi was sometimes not detected by culture of nasopharyngeal swabs from carriers for up to 2 or 3 months before nasal shedding resumed sporadically. The study found that PCR was a more sensitive technique for detecting S. equi on swabs: many more known positive swabs were detected using PCR than using culture (56 of 61 swabs positive by PCR vs. 18 of 61 swabs positive by culture). Similar results were obtained for guttural pouch samples from 12 established carriers (PCR 76% vs. culture 59%). PCR also allows differentiation of the two subspecies, equi and zooepidemicus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of S. equi infection.
  • Help ensure that horse populations are free of S. equi
  • Early prevention of spread of this bacterium
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products that derive from horses

References:
Chanter, N. (1997) Streptococci and enterococci as animal pathogens. J. Appl. Microbiol. Symp. Suppl. 83:100S-109S.
Newton, J.R., Verheyen, K., Talbot, N.C., Timoney, J.F., Wood, J.L., Lakhani, K.H. and Chanter, N. (2000) Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and culture of Streptococcus equi. Equine Vet J. 32:515-526.

Specimen requirements: Nasopharyngeal swab, or lymph node abscess swab, or gutteral pouch swab, or 0.2 ml fresh, frozen or fixed tissue, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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