Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis


Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii



Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel



Enterobacter cloacae


Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Francisella tularensis




Hepatitis E

Herring worms


Influenza type A

Influenza type B

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses


Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia


Pseudoterranova worms

Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi



Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Japanese encephalitis PCR test
wildlife and zoo assay data sheet

Japanese encephalitis

Test code:
S0062 - Ultrasensitive qualitative detection of Japanese encephalitis virus by reverse transcription coupled real time polymerase chain reaction


One of the leading causes of acute encephalopathy in children in the tropics is Japanese encephalitis (JE). An arbovirus, the Japanese encephalitis virus is transmitted by Culex mosquitoes and is a member of genus Flavivirus of the family Flaviviridae. The RNA genome of the JE virus is positive sense, single-stranded, approximately 11 Kb in length, and contains one long open reading frame.

JE virus is related to Murray Valley encephalitis virus. The virus is neurotropic and predominately affects the thalamus, anterior horns of the spinal cord, cerebral cortex, and cerebellum. It mainly affects children <15 years of age and is mostly asymptomatic. The occasional symptomatic child typically presents with a neurological syndrome characterized by altered sensorium, seizures, and features of intracranial hypertension. Though no antiviral drug is available against JE, effective supportive management can improve the outcome. Control of JE involves efficient vector control and appropriate use of vaccines.

Horses and primates can develop similar pathological lesions to those in human when infected with JE virus; they are considered dead-end hosts for JE. Most horses infected by JE virus show mild clinical signs, including fever, anorexia and depression. However, the mortality rate is high when JE infected horses show neurological symptoms (Ihara et al., 1997). Seroepidemiological survey of Asian monkeys has also shown widespread infection of these primates with JE virus (Yuwono et al., 1984). The virus can also infect birds, pigs and donkeys. Currently, the virus is mainly detected in East Asia, southeast Russia, India, Papua New Guinea and the Torres Strait Islands.

Conventional methods of JE diagnosis, including hemagglutination-inhibition and complement fixation tests for antibody assay, have been ineffective because of low sensitivity. Although a new immunoassay was developed to detect earlier, virus-specific IgM antibodies in the serum and cerebrospinal fluid (CSF) of acute and convalescent-phase patients, this new assay also suffered from low sensitivity and non-specific reaction. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to detect Flavivirus rapidly and specifically.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of JE infection.
  • Help ensure that animal populations are free of JE virus
  • Early prevention of spread of the virus
  • Minimize human exposure to the virus

Ihara, T., Kano, R., Nakajima, Y., Sugiura, T., Imagawa, H., Izuchi, T. and Samjima, T. (1997) Detection of antibody to Japanese encephalitis virus (JEV) by enzyme-linked immunosorbent assay (ELISA). J. Equine Sci. 8: 25-28.
Yuwono, J., Suharyono, W., Koiman, I., Tsuchiya, Y. and Tagaya, I. (1984) Seroepidemiological survey on dengue and Japanese encephalitis virus infections in Asian monkeys. Southeast Asian J Trop Med Public Health. 15:194-20

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh or frozen CNS tissue, or 0.2 ml CSF, serum or plasma.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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