wildlife and zoo assay data sheet
Ultrasensitive qualitative detection of turtle fraservirus by
reverse transcription coupled real time PCR
Turtle fraservirus 1 (TFV-1) was recently isolated from wild freshwater
turtles in Florida (Waltzek et al., 2022). This virus is a
negative-sense RNA virus composed of two RNA segments, the L
segment and the S segment. A new family, Tosoviridae, has been
proposed for this virus to commemorate the discovery of the
virus in the Tosohatchee Wildlife Management Area.
This virus has been confirmed to be able to infect Florida softshell
turtles, as well as Florida’s cooter
species (genus Pseudemys),
eastern snapping turtles (Chelydra
serpentina), and red-eared sliders (Trachemys
Infected turtles show weakness, lethargy, and swollen, closed or
sunken eyelids. There is discharge from the nose, eyes and
discoloration of the shell. Occasionally, infected turtles show
difficulty in breathing, reluctance to flee and irregular
swimming. Many infected turtles also develop secondary microbial
infections and vascular injury from spirochiidiasis. Currently
the transmission route is not clear.
Culture detection of the virus is not specific and not sensitive.
Diagnosis of this viral infection is more effective using
molecular detection of the virus by PCR.
Help confirm the disease causing agent
Identify carriers of this virus
Help ensure that animal colonies and wild populations are free of
Early prevention of spread of this virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
Waltzek, T.B. et al. A novel group of negative-sense RNA viruses
associated with epizootics in managed and free-ranging
freshwater turtles in Florida, USA. PLOS Pathogens, March 11,
Nasal swab, or eye swab, or cloacal swab, or oropharyngeal swab, or 0.2
ml fresh or frozen tissue, or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real time PCR