Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis


Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii



Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel



Enterobacter cloacae


Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Francisella tularensis




Hepatitis E

Herring worms


Influenza type A

Influenza type B

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses


Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia


Pseudoterranova worms

Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi



Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Vesicular stomatitis PCR test
wildlife and zoo assay data sheet

Vesicular stomatitis virus (VSV)

Test code: S0068 - Ultrasensitive qualitative detection of vesicular stomatitis virus by reverse transcription coupled real time polymerase chain reaction. This assay detects and differentiates the New Jersey and Indiana serotypes of the vesicular stomatitis virus.


Vesicular stomatitis (VS) is a disease of horses, cattle, swine, sheep, goats and deer caused by related viruses in the genus Vesiculovirus of the family Rhabdoviridae. The viral genome is 11 kb and codes for five main proteins: the nucleocapsid (N), phosphoprotein (P), matrix (M), glycoprotein(G), and polymerase (L). Serologically, VS viruses have been classified into two main serotypes: New Jersey (NJ) and Indiana (IN). In addition to the classic VSV-IN (IN1), two additional subtypes, Cocal virus (VSV-IN2) and Alagoas virus (VSV-IN3), have been described.

VS is prevalent in northern South America, throughout Central America, and at one site in the United States: Ossabaw Island, Georgia. However, outbreaks of VS have been reported in the western United States, northern Mexico, and temperate regions of South America. The disease can cause blisters on an animal's mouth, teats or hooves. Clinical outbreaks of VS in livestock can result in significant economic impact on producers and extensive regulatory responses by governmental agencies, including trade restrictions, market closures, and quarantines. Such measures are needed because of the clinical similarities between VS and foot-and-mouth disease and other foreign vesicular diseases in livestock species.


  • Help confirm the disease causing agent
  • Distinguish between VS and other vesicular diseases including foot-and-mouth
  • Shorten the time required to confirm a clinical diagnosis of VS infection
  • Help ensure that animal herds and populations are free of VS
  • Early prevention of spread of VS
  • Minimize human exposure to the virus

Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A., Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003) A single-tube multiplex reverse transcription-polymerase chain reaction for detection and differentiation of vesicular stomatitis Indiana 1 and New Jersey viruses in insects. J. Vet. Diagn. Invest. 15:561-567.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or lesion swab or nasopharyngeal swab, or 0.2 ml nasal wash, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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