wildlife, zoo and aquatic assay data sheet
Ultrasensitive qualitative detection of Pseudoloma
neurophilia by real time
neurophilia, a microsporidian parasite, is the most
prevalent pathogen found in zebrafish (Danio rerio)
research facilities. Infections occur primarily in the spinal
cord and are associated with emaciation and scoliotic changes.
It was initially discovered as an unusual xenoma-forming
microsporidium in the central nervous system of moribund
zebrafish from a laboratory colony in Eugene, Oregon.
Spores of this
parasite are uninucleate, ovoid to pyriform, with a prominent
posterior vacuole. The spores are average 5.4 × 2.7 μm in size
with 13–16 coils of the polar filament. Infection with this
parasite can produce xenomas within the spinal cord and
hindbrain. The xenomas usually contain sporophorous vesicles
with up to 16 spores. Sporoblasts and presporoblast stages
(probably sporonts) are found occasionally in small aggregates
dispersed randomly throughout the xenomas.
can also be found in the ovaries of infected fishes. Vertical
transmission appears to help spread the parasite through a
population. The sharing of various strains of zebrafish among
research facilities can spread the parasites to other research
traditional diagnostic methods, PCR diagnosis is rapid, specific
and highly sensitive. It allows for screening of large numbers
of fish, and can be applied to eggs, water filtrates, biofilms,
and other sample types (Whipps and Kent, 2006.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of this parasite
Help ensure that zebrafish facilities are free of this
Early prevention of spread of this parasite in a facility
or geographic area
Minimize human exposure to this parasite
Safety monitoring of biological products that derive
from susceptible animals
C.M. and Kent, M.L. (2006) Polymerase Chain Reaction Detection
of Pseudoloma neurophilia, a Common Microsporidian of Zebrafish
(Danio rerio) Reared in Research Laboratories. J. Am. Assoc.
Lab. Anim. Sci. 45: 36–39.
Skin or vent swab, or biofilm swab, or filter media,
or 10 ml
water, or 0.2 ml tissue, or 0.2 ml culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR