Bordetella PCR test
dog and cat assay data sheet
Bordetella bronchiseptica
Test code:
B0046 -
Ultrasensitive qualitative detection of
Bordetella bronchiseptica
by real time PCR
B0046 is included
in P0019 - canine respiratory
panel and in P0020 - feline
respiratory panel
Infection by
Bordetella bronchiseptica
is an important cause of morbidity and mortality in some common
domestic animals, including dogs, pigs and rabbits. Infection of
horses, seals, cats and humans have also been reported. In dogs,
infectious tracheobronchitis is caused by
B. bronchiseptica, canine
adenovirus-2, and canine parainfluenza virus, or by co infections by
combinations of these organisms. Infection can result in acute or
chronic cough, ciliary impairment, local respiratory immunosuppression,
and predisposition to secondary lower-respiratory infections. In
animal shelters, this "kennel cough" in dogs represents a major
management problem; it is readily transmissible, reduces adoption
rates for affected animals, and requires intensive medical management.
Dogs with infectious tracheobronchitis are euthanized in some
shelters.
Tracheobronchitis,
conjunctivitis and rhinitis (upper-respiratory tract disease),
mandibular lymphadenopathy, and pneumonia have been reported in cats
infected with B. bronchiseptica.
Positive identification of B.
bronchiseptica in cats is absolutely required to institute
the appropriate treatment because upper-respiratory infection (URI) in
cats can also be caused by herpesvirus, calicivirus,
Mycoplasma species, and
Chlamydia psittaci
infection. It is believed that many cats could be carriers of the
bacteria because many B.
bronchiseptica culture-positive cats are clinically
normal. When these cats are co-infected with other pathogens, URI will
develop. Although it is not known how likely cats are to infect dogs
with B. bronchiseptica,
putative cross-species transmission of
B. bronchiseptica has been
described.
Timely detection
of B. bronchiseptica
is very important for surveillance of the disease and control of the
bacterial infection. Early detection can be crucial to successful
treatment of the disease with antibiotics and to control the spread of
the disease. The conventional method of detection, by culture, is
specific but lacks sensitivity. It also takes 3 to 7 days to obtain a
result. Current serological tests do not differentiate the closely
related Bordetella
species. Only molecular detection by PCR can give both rapid and
specific identification of B.
bronchiseptica.
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of Bordetella
bronchiseptica
-
Help ensure that animal facilities are free of
B. bronchiseptica
-
Early prevention of spread of
B. bronchiseptica
among a facility
-
Minimize human exposure to
B. bronchiseptica
-
Safety monitoring of biological products that derive
from susceptible animals
Specimen
requirements:
Nasopharyngeal swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
information.
Turnaround
time:
2 business days
Methodology:
Qualitative real time
PCR
Normal range:
Nondetected
Bordetella PCR test
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