F0004 - Ultrasensitive qualitative detection of
by real time PCR
is an opportunistic airborne pathogen capable of causing
life-threatening pneumonia in AIDS patients and other
immunocompromised individuals. While humans can be carriers,
P. carinii is also
found in a wide variety of other mammalian hosts worldwide.
Using molecular techniques,
P. carinii DNA has been detected in air samples,
asymptomatic animals, normal healthy individuals and
immunocompetent patients with other diseases.
historically was considered a protozoan but has recently been
reclassified as a fungus based on nucleic acid sequence
information. The organism does not grow well in culture, making
it difficult to study or detect diagnostically by that means.
The organism can cause fatal pneumonia in immunodeficient
animals (e.g. SCIDs and athymic nude mice), and in
immunologically competent animals that are on an
this pathogen occurs widely in the environment, many animals
have been exposed to it and serology testing is therefore unable
to reliably determine animals’ current carrier status. Detection
of this pathogen by PCR is useful because this technique is very
sensitive and specific. Results will not be compromised if the
infection is at early stage or the animal is immunodeficient.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
Help ensure that animal facilities are free of
Early prevention of spread of
P. carinii among
Minimize personnel exposure to
Safety monitoring of biological products that derive
from affected animals
Cushion, M.T., Kaselis, M., Stringer, S.L. and Stringer, J.R.
(1993) Genetic stability and Diversity of Pneumocystis carinii
Infecting Rat Colonies. Infection and Immunity, 61: 4801-4813.
Nasal swab, or 0.2 ml lung tissue, or 0.2 ml bronchoalveolar
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time polymerase chain reaction