RD114 virus PCR test
dog and cat assay data sheet
S0240 - Ultrasensitive qualitative detection of feline endogenous
virus RD114 by reverse transcription coupled real time polymerase chain reaction
RD-114 virus is a retrovirus discovered in 1971 from a human tumor cell
line (RD-114 cells). This cell line was derived from a human
rhabdomyosarcoma after passage through fetal cats. Sequence analysis
of the virus reveals that the virus is an endogenous retrovirus that
has been incorporated in the feline genome. It is therefore not a
human exogenous retrovirus.
All domestic cats are assumed to have the RD-114 viral genome integrated.
Although reactivation of the endogenous virus is not common, several
feline cell lines have been shown to produce RD-114-related viruses.
For example, a widely used feline cell line, Crandell-Rees feline
kidney (CRFK) cells, actively produces infectious RD-114 viral
particles. Recent studies have found that certain live attenuated
vaccines for dogs are contaminated with infectious RD114 virus, which
casts worries about the biological safety of these vaccines. Although
experimental infection of dogs with RD-114 does not cause any
significant immediate clinical symptoms to the dogs, long term effects
have not been fully characterized.
Detection of this virus is currently by PCR, which offers high
sensitivity and specificity.
Detect the presence of this virus in cell lines and veterinary
Detect the presence of this virus in susceptible animals
Narushima, R., Shimazaki, T. and Takahashi, T. (2011) Development of a
real-time reverse-transcription-PCR method for detection of RD114
virus in canine vaccines. Biologicals. 39:89-93
requirement: 0.2 ml
cell culture or vaccine, or 0.2 ml whole blood in EDTA (purple top)
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative reverse transcription coupled real time PCR