Top dogs can catch things too!  Our NEW dog show panel checks for 8 pathogens potentially transmissible at dog shows.

 Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 8 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella canis

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine circovirus

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine pneumovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

Demodex gatoi mites

E. coli

Ehrlichia

Entamoeba

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza type A

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

Mange in cats

Microsporum

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma cynos

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neorickettsia helmintheca

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

RD114

Reovirus screen

Rickettsia screen

Ringworm

Salmonella

Salmon poisoning disease

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trichophyton

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


RD114 virus PCR test

dog and cat assay data sheet

RD-114 virus

Test code:
S0240 - Ultrasensitive qualitative detection of feline endogenous virus RD114 by reverse transcription coupled real time polymerase chain reaction

RD-114 virus is a retrovirus discovered in 1971 from a human tumor cell line (RD-114 cells). This cell line was derived from a human rhabdomyosarcoma after passage through fetal cats. Sequence analysis of the virus reveals that the virus is an endogenous retrovirus that has been incorporated in the feline genome. It is therefore not a human exogenous retrovirus.

All domestic cats are assumed to have the RD-114 viral genome integrated. Although reactivation of the endogenous virus is not common, several feline cell lines have been shown to produce RD-114-related viruses. For example, a widely used feline cell line, Crandell-Rees feline kidney (CRFK) cells, actively produces infectious RD-114 viral particles. Recent studies have found that certain live attenuated vaccines for dogs are contaminated with infectious RD114 virus, which casts worries about the biological safety of these vaccines. Although experimental infection of dogs with RD-114 does not cause any significant immediate clinical symptoms to the dogs, long term effects have not been fully characterized.

Detection of this virus is currently by PCR, which offers high sensitivity and specificity.

Utilities:

  • Detect the presence of this virus in cell lines and veterinary vaccines
  • Detect the presence of this virus in susceptible animals

References:
Narushima, R., Shimazaki, T. and Takahashi, T. (2011) Development of a real-time reverse-transcription-PCR method for detection of RD114 virus in canine vaccines. Biologicals. 39:89-93

Specimen requirement: 0.2 ml cell culture or vaccine, or 0.2 ml whole blood in EDTA (purple top) tube

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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