dog and cat assay data sheet

Canine parainfluenza virus (CPIV)

Test code: S0109 - Ultrasensitive qualitative detection of canine parainfluenza virus by reverse transcription coupled real time polymerase chain reaction.

S0109 is included on P0019 - canine respiratory panel

Canine parainfluenza virus (CPIV) is an important cause of kennel cough in the USA and is commonly isolated from outbreaks of the disease. It has also been identified in other parts of the world. Experimentally, the disease is only very mild or subclinical. However, in the field CPIV infection may predispose dogs to combined infections with other viral and/or bacterial agents, leading to more typical signs of kennel cough.

The virus replicates mainly in the epithelial cells of the nasal mucosa, pharynx trachea, large bronchi, and regional lymph nodes. Generalized infection does not occur, except in immunocompromised animals.

CPIV can be detected by cell culture but this method is not very sensitive. Molecular detection of PCR is the most rapid, sensitive and specific method for identifying this virus.

Utilities:

• Confirm the disease causing agent
• Ensure that animal groups and populations are free of CPIV
• Early prevention of spread of CPIV among a population
• Minimize human exposure to this virus
• Safety monitoring of biological products and vaccines that derive from susceptible animals

Specimen requirement: Nasopharyngeal swab or 0.5 ml tracheal wash.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected