NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

            * * *           

Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Lawsonia PCR test for dogs and cats

dog and cat assay data sheet

Lawsonia intracellularis

Test code:
B0035 - Ultrasensitive qualitative detection of Lawsonia intracellularis by real time polymerase chain reaction.

B0035 is included on P0022 - canine diarrhea panel

 

Proliferative enteropathy, also known as proliferative ileitis, is caused by infection with Lawsonia intracellularis, an obligate intracellular, curve-shaped, argyrophilic bacterium. The disease has been detected in domestic and laboratory animals including primates, pig, horse, dog, rat, ferret, guinea pig, rabbit and hamster. The disease has been reported sporadically in foals 3-7 months of age and one outbreak involving 3 different breeding farms has been described. All reported cases were in the eastern half of Canada or the United States. The swine industry suffers the most significant impact from this disease. The disease has two clinical manifestations in pigs: an acute hemorrhagic form often called porcine hemorrhagic enteropathy, and a more chronic proliferative form often referred as porcine intestinal adenomatosis.

Environmental contamination with feces of infected animals appears to be the most important route of transmission of disease, but it is currently unknown how long Lawsonia intracellularis can remain infectious outside the animal. Infection occurs most often during the post-weaning period, when passive maternal immunity declines. After ingestion, the bacteria infect intestinal proliferating crypt epithelial cells and multiply within the apical cytoplasm. There is no evidence of infection of tissues other than intestine. Most infected animals have subclinical infection but shed the bacteria in their feces, leading to environmental contamination. Clinical manifestation of an infection can be triggered by stressors, such as overcrowding, transport, change in diet, and experimental manipulation.

Bacterial culture and isolation of the bacteria are difficult because cultured enterocytes are required to support the growth of Lawsonia intracellularis. Electron microscopy can be used to detect the curved bacterial rods in apical cytoplasm of enterocytes but this is a very time-consuming process and few laboratories have such capability. Immunohistochemical detection can be performed on formalin-fixed, paraffin-embedded tissue from affected intestine to detect the bacteria in following biopsy or necropsy, but this is also a very time-consuming process. Molecular detection using PCR is the most sensitive, rapid and specific method to confirm presence of the Lawsonia intracellularis genome within tissue samples (Jones et al., 1993).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Lawsonia infection.
  • Help ensure that animal populations are free of Lawsonia
  • Early prevention of spread of this bacterium
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products that derive from susceptible animals

References:
Jones, G. F., Ward, G.E., Murtaugh, M.P., Lin, G. and Gebhart, C.J. (1993) Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis in feces by polymerase chain reaction. J. Clin. Microbiol. 31:2611-2615.

Specimen requirements: 0.2 ml feces, or rectal swab, or 0.2 ml fresh, frozen or fixed ileum tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2016 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880