NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Influenza PCR test for canine and feline

dog and cat assay data sheet

Influenza

Test code: S0077 - Ultrasensitive qualitative detection of influenza A virus by reverse transcription coupled real time polymerase chain reaction. This assay detects but does not differentiate most known strains of influenza A viruses, including H5N1, H5N2, H1N1, H2N2, H3N8, H4N6, H7N7, H8N4 and H9N2.

S0077 is included on P0019 - canine respiratory panel and on P0020 - feline respiratory panel

 

Influenza is a severe acute upper respiratory infection, and typical symptoms include pyrexia, dyspnea, anorexia and coughing.

Several different subtypes and strains of influenza viruses infect humans, cats, dogs, swine, birds, horses and other animals. Among these different strains, the canine influenza virus (H3N8) has only recently emerged, with the first recognized outbreak occurring on a racetrack in Florida in January 2004. Zoologix assay S0077 detects canine influenza but does not differentiate it from other influenza strains also detected by this assay. Dogs are not usually susceptible to most other influenza viruses; however, an unpublished study carried out in 2005 by the National Institute of Animal Health in Bangkok indicated that dogs could be infected with avian influenza virus, although no associated disease was detected.

Genetic differences among influenza viruses normally impede cross-species infection. However, a study published in September 2004 demonstrated that domestic cats can become infected with the H5N1 avian influenza virus and are capable of transmitting the virus to other cats (http://www.avma.org/public_health/influenza/avian_faq.asp; http://www.cdc.gov/flu/avian/gen-info/qa.htm ). In February 2006, authorities in Germany reported that a domestic cat had died from H5N1 avian influenza. That cat lived in the northern island of Ruegen, where more than 100 wild birds are believed to have died of the disease, and it likely contracted the disease by eating one of those infected birds. In March 2006, three cats in Austria were confirmed to be ill with the H5N1 virus. These cats were among 170 living in an animal shelter where the disease had been detected in chickens a month earlier. Prior to confirmation of these feline cases, there had been anecdotal reports of H5N1 infection in domestic cats in southeast Asia and Iraq.

Large cats kept in captivity can be infected with avian influenza as well. In December 2003, two tigers and two leopards that were fed fresh chicken carcasses from a local slaughterhouse died at a zoo in Thailand. Avian influenza virus H5N1 was identified in their tissue samples. In February and March 2004, the virus was detected in a clouded leopard and white tiger, respectively, both of which died in a zoo near Bangkok. In October 2004, 147 of 441 captive tigers in a zoo in Thailand died or were euthanatized as a result of infection after being fed raw chicken carcasses. Results of a subsequent investigation suggested that at least some tiger-to-tiger transmission occurred in that facility.

Because the spread of influenza viruses is airborne, one infected animal can quickly spread influenza to other animals with only casual contact. Rapid testing of suspected influenza cases is thus essential to control outbreaks of the disease.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of influenza
  • Help ensure that animal populations are free of influenza
  • Early prevention of spread of this virus
  • Minimize personnel exposure to this virus

References:
Daly, J. M., Lai, A.C.K., Binns, M.M., Chambers, T.M., Barrandeguy, M. and Mumford, J.A. (1996) Antigenic and genetic evolution of equine H3N8 influenza A viruses. J. Gen. Virol. 77:661-671.
Mumford, J. A., and Wood, J.M. (1993) WHO/OIE meeting: consultation on newly emerging strains of equine influenza. Vaccine 11:1172-1175.
Office International des Epizooties (OIE) (2000) Equine influenza, p. 546-557. In Manual of standards for diagnostic tests and vaccines. OIE, Paris, France.
Quinlivan, M., Cullinane, A., Nelly, M., Van Maanen, K., Heldens, J. and Arkins, S. (2004) Comparison of sensitivities of virus isolation, antigen detection, and nucleic acid amplification for detection of equine influenza virus. J. Clin. Microbiol. 42:759-763.
van Maanen, C. and Cullinane, A. (2002) Equine influenza virus infections: an update. Vet. Q. 24:79-94.
Webster, R. G. (1993) Are equine 1 influenza viruses still present in horses? Equine Vet. J. 25:537-538.

Specimen requirements:

Preferred samples: tracheal swab or nasopharyngeal swab.

Less preferred sample: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions. For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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