- Ultrasensitive qualitative detection of Streptococcus pyogenes by
real time polymerase
Streptococcus pyogenes is a gram-positive
spherical-shaped bacterium. It is beta-hemolytic and belongs to
Lancefield serogroup A, also known as group A streptococci (GAS).
The bacteria can cause a wide variety of diseases in humans
such as acute pharyngitis, accounting for 15%-30% of cases in children
and 5%-10% of cases in adults. During the winter and spring
in temperate climates, up to 20% of asymptomatic school-aged children
may be GAS carriers.
Although GAS usually causes
pharyngitis or impetigo, it can infrequently cause invasive diseases
such as cellulitis, bacteremia, necrotizing fasciitis and toxic shock
syndrome (TSS). Along with Staphylococcus aureus, GAS is one of the
most common pathogens responsible for cellulitis.
is ubiquitous in nature and many animal species are carriers of these
bacteria. Close contact between
and humans can spread this infection in both directions.
Although culture may be used to detect these bacteria, molecular
detection by PCR is a rapid, highly sensitive and specific method
(Bexiga et al., 2011).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of S.
Help ensure that colonies are free of S. pyogenes
Early prevention of spread of this bacterium
Minimize personnel exposure to this bacterium
Safety monitoring of biological products that derive from primates
0.2 ml whole blood
in EDTA (purple top) or ACD (yellow top) tube, or serum, or bacterial
culture; or nasal, pharyngeal or oral swabs.
For specimen types other than those listed here, please call to
confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain frozen
in transit. See
shipping instructions for more information.
Turnaround time: 2 business days
Qualitative real time PCR
Normal range: Nondetected