Chlamydiosis PCR test for cats
dog and cat assay data sheet
Chlamydophila felis (formerly
Chlamydophila psittaci / Chlamydia psittaci),
pneumonitis" or "feline chlamydiosis"
B0034 - Qualitative detection of
Chlamydophila felis bacteria by polymerase chain reaction
B0034 is included
on P0020 - feline respiratory
chlamydiosis, also known as feline pneumonitis, is a relatively mild,
chronic upper respiratory disease caused by the bacterium
also known as C. psittaci.
The major symptom is conjunctivitis which is an abnormal eye discharge
due to inflammation of the conjunctiva, the membrane lining the inside
of the eyelid. The infection can also cause nasal discharge, sneezing,
and pneumonia. If infected cats are left untreated, the infection
tends to become chronic, lasting weeks or months.
part of the feline Upper Respiratory Infection (URI) complex which
includes a group of viral and bacterial infections (eg feline
rhinotracheitis, feline calicivirus) that affect the nose and eyes,
manifesting similar symptoms. Chlamydiosis accounts for about 10% to
15% of all feline URI cases and often occurs with another URI.
occurs in about 5% to 10% of the cat population worldwide. It is
especially common in kittens 2 to 6 months old, in multicat
households, and in pet adoption shelters. Outbreaks tend to occur in
overcrowded, poorly ventilated and unsanitary settings, and where cats
are poorly fed or stressed, either physically (eg extreme
temperatures) or psychologically (eg introduction of a new cat in a
Many infected cats
have very mild symptoms and they become carriers of
C. felis. These carrier cats often shed the bacteria in their
eye discharge. The likelihood that bacteria will be present in the
discharge is greater after stressful events. Though uncommon, cases
have been reported of mild human conjunctivitis caused by
clinical symptoms of chlamydiosis are similar to those of other feline
upper respiratory infections, it is important to accurately identify
the bacteria before successful treatment can be administered.
Traditionally culture was used, but it is very difficult to
differentiate by culture C. felis from other bacteria also found in eye discharge.
Immunofluorescent staining has also been used to identify
C. felis, but this
technique’s sensitivity is also not high. However, molecular detection
by PCR is a rapid, sensitive and specific method to detect this
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of C. felis
Help ensure that feline populations are free of
Early prevention of spread of this bacterium among a cat
Minimize human exposure to this bacterium
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Chlamydiosis PCR test for cats