Cronobacter sakazakii (formerly
- Ultrasensitive qualitative detection of Cronobacter
sakazakii by real time polymerase chain reaction.
is ubiquitously present in the environment and is one of the
most common opportunistic pathogens. It can be pathogenic in
patients with low immune responses, such as newborn infants, the
elderly, and cancer patients. The bacteria can contaminate many
C. sakazakii is Gram-negative, with a rod shaped body
structure approximately 3 by 1 micrometers. It is highly
flagellated (peritrichous) and can produce a protective biofilm,
allowing it to adhere firmly to surfaces such as silicon, PVC,
polycarbonate, glass, stainless steel and interior surfaces of
public water systems. Contamination of medical devices by this
organism is very hard to remove.
In humans infected with C. sakazakii, the vascular,
gastrointestinal and nervous systems are especially affected.
Establishment of these bacteria in the human vascular system
causes bacteremia and/or sepsis. The bacteria may eventually
infect the central nervous system causing meningitis,
intra-cerebral infarctions, brain abscess and/or cyst formation.
Necrotizing enterocolitis (NEC) is also associated with C.
sakazakii infection, and is the most common
gastrointestinal emergency in neonates. Once infected, neonates
can have a mortality rate of 40 to 80%, and a 20% chance that
the surviving patient may develop serious neurological
complications such as hydrocephalus, quadriplegia, brain abscess
and retarded neural development.
Although biochemical characterization can be used to identify
these bacteria, it is both time consuming and labor intensive.
Alternatively, molecular detection by PCR is highly specific,
sensitive and fast (Kang et al., 2007).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of
C. sakazakii infection
Help ensure that animal populations and colonies are free of
Early prevention of spread of C.
Minimize personnel exposure to C.
Safety monitoring of food
Safety monitoring of biological products that derive from
Kang, E.S., Yong, S.N. and Kwang, W.H. (2007)
Rapid detection of Enterobacter sakazakii using TaqMan real-time
PCR assay. J. Microbiol. Biotechnol. 17:516-519.
ml whole blood in EDTA (purple top) or ACD (yellow top) tube,
or environmental swab, or food swab, or 0.2 ml urine, or 0.2 ml CSF, or 0.2 ml feces, or
fecal swab, or nasopharyngeal swab.
For specimen types other than those listed here, please call to
confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Qualitative real time PCR
Normal range: Nondetected