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Cronobacter sakazakii PCR test for primates
primate assay data sheet

Cronobacter sakazakii (formerly Enterobacter sakazakii)

Test code:

B0090 - Ultrasensitive qualitative detection of Cronobacter sakazakii by real time polymerase chain reaction.

Cronobacter sakazakii is ubiquitously present in the environment and is one of the most common opportunistic pathogens. It can be pathogenic in patients with low immune responses, such as newborn infants, the elderly, and cancer patients. The bacteria can contaminate many foods.

C. sakazakii is Gram-negative, with a rod shaped body structure approximately 3 by 1 micrometers. It is highly flagellated (peritrichous) and can produce a protective biofilm, allowing it to adhere firmly to surfaces such as silicon, PVC, polycarbonate, glass, stainless steel and interior surfaces of public water systems. Contamination of medical devices by this organism is very hard to remove.

In humans infected with C. sakazakii, the vascular, gastrointestinal and nervous systems are especially affected. Establishment of these bacteria in the human vascular system causes bacteremia and/or sepsis. The bacteria may eventually infect the central nervous system causing meningitis, intra-cerebral infarctions, brain abscess and/or cyst formation. Necrotizing enterocolitis (NEC) is also associated with C. sakazakii infection, and is the most common gastrointestinal emergency in neonates. Once infected, neonates can have a mortality rate of 40 to 80%, and a 20% chance that the surviving patient may develop serious neurological complications such as hydrocephalus, quadriplegia, brain abscess and retarded neural development.

Although biochemical characterization can be used to identify these bacteria, it is both time consuming and labor intensive. Alternatively, molecular detection by PCR is highly specific, sensitive and fast (Kang et al., 2007).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of C. sakazakii infection
  • Help ensure that animal populations and colonies are free of C. sakazakii
  • Early prevention of spread of C. sakazakii
  • Minimize personnel exposure to C. sakazakii
  • Safety monitoring of food
  • Safety monitoring of biological products that derive from susceptible animals

References:
Kang, E.S., Yong, S.N. and Kwang, W.H. (2007) Rapid detection of Enterobacter sakazakii using TaqMan real-time PCR assay. J. Microbiol. Biotechnol. 17:516-519
.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or environmental swab, or food swab, or 0.2 ml urine, or 0.2 ml CSF, or 0.2 ml feces, or fecal swab, or nasopharyngeal swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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