- Qualitative detection of baboon CMV by polymerase chain
Cytomegalovirus belongs to the beta subgroup of the
and is a ubiquitous agent in many species. CMV in baboon was
first isolated in 1996 and has been shown to be related to CMVs
of other primate species both genetically and antigenically,
particularly to those from drills and macaques.
Similar to CMV in humans and macaques, almost all adult baboons
are seropositive for CMV, and a high percentage of them are
latent carriers shedding virus continually in urine and saliva.
If the infected individual is immunocompromised, a high level of
virus secretion is expected.
In a typical baboon breeding colony, CMV is readily transmitted
among infants and juveniles. By 2.5 years of age, more than 95%
of them are seropositive (Payton et al., 2004).
Diagnosis of active infection of
animals with CMV can be achieved using cell culture, but cell
culture has low sensitivity. Serological methods are not
suitable because most baboons are seropositive for CMV.
Molecular detection by PCR can provide highly sensitive and
specific detection of the virus in a rapid manner.
Help confirm the disease causing agent
Help ensure that animal colonies are free of this virus
Early prevention of spread of this virus among a colony
Minimize personnel exposure to this virus
Safety monitoring of biological products and vaccines that
derive from primates
M.E., d'Offay, J.M., Prado, M.E., Black, D.H., Damania, B.,
White, G.L. and Eberle, R. (2004) Comparative transmission of
multiple herpesviruses and simian virus 40 in a baboon breeding
colony. Comp. Med. 54:695-704.
Oral swab, or 0.2 ml urine, or 0.2 ml whole blood in EDTA
(purple top) tube, or 0.2 ml serum or
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for more information.
2 business days