primate genetic test data sheet

Fetal sex determination for macaques by PCR

Test code: GP004

Nonhuman primates have reproductive characteristics, placental structures and development events very similar to those of humans. Thus, primates are often used in studies of fetal development, or drug-drug interactions during fetal development.

Non-invasive fetal sex determination is necessary for some biomedical studies because of differences during male and female fetal development. The male phenotype is controlled by the sex-determining region Y gene (SRY gene). This gene is on the Y chromosome, which is not present in females. Therefore, detection of this gene target in fetal DNA circulating in maternal blood is a non-invasive indicator of a male fetus (Mitsunaga et al., 2010).

Utilities:

• Determine macaque fetal sex from a maternal blood sample

References:
Mitsunaga, F., Ueiwa, M., Kamanaka, Y., Morimoto, M. and Nakamura, S. (2010) Fetal sex determination of macaque monkeys by a nested PCR using maternal plasma.  Exp. Anim. 59:255-260.

Specimen requirement: 0.2 ml fresh or frozen EDTA maternal whole blood, or 0.2 ml frozen maternal plasma or serum

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 3 business days

Methodology: PCR