ultrasensitive detection of
bacteria by real time polymerase chain reaction. This assay
detects but does not differentiate most serotypes of
Test B0040 is
- waterborne pathogens screening panel
are gram negative bacteria that occur worldwide, inhabiting the
intestinal tracts of many species including humans, nonhuman
primates, birds, horses, pigs, dogs, cats, rats, mice, hamsters,
guinea pigs and other species. The house mouse is of special
concern, as it is often a carrier and plays a significant role
in spreading human and animal salmonellosis.
more than 1600 recognized serotypes of
these, S. typhimurium
and S. enteritidis
have been associated most commonly with lab animal colony
infections. Some shipments of rhesus and cynomolgus monkeys have
been found to include 20% or more
a recent report (http://www.defra.gov.uk/corporate/vla/science/
documents/science-end-survrep-qtlyw405.pdf), garden bird
salmonellosis is the most frequently diagnosed infectious
disease among the wild birds submitted to the United Kingdom’s
Diseases of Wildlife scheme. The condition is primarily caused
by infection with Salmonella
typhimurium, although other serotypes have been
reported. Environmental contamination by these infected birds
can lead to the exposure of other species, notably cats, horses
and humans. Animal feed containing contaminated animal
byproducts continues to be a source of
infection, especially in diets containing raw, unpelleted meal.
animals infected by
Salmonella bacteria can be asymptomatic, with
clinical signs precipitated by stress. Clinical infections may
progress to enteritis, septicemia and variable mortality. A high
percentage of survivors become carriers. In humans, acute
gastroenteritis can result in sudden onset of abdominal pain,
diarrhea, nausea, and fever. Septicemia may sometimes develop in
humans, especially in immunocompromised patients.
blood culture with selective media have been used to identify
Salmonella. However, identification by culture has relatively
low sensitivity and is quite slow. Molecular detection of these
bacteria, on the other hand, is a highly sensitive and specific
technique, and is also more tolerant of suboptimal specimen
shipping and handling conditions.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Salmonella
Help ensure that colonies are free of these bacteria
Early prevention of spread of these bacteria among a
Minimize personnel exposure to these bacteria
Safety monitoring of biological products and vaccines
that derive from primates
- rectal swab, or cloacal swab, or 0.2 ml feces or bacterial
culture, or environmental swab.
- 0.2 ml whole blood in EDTA (purple top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR