- Ultrasensitive qualitative screen for
reoviruses by reverse
transcription coupled real time PCR. This
assay detects a wide range of mammalian
reoviruses but does not detect
are comprised of 10 to 12 double-stranded RNA genomic segments
that can reassort both in nature and in laboratory settings. The
most common mammalian isolates are type 1 (Lang), type 2
(Jones), and type 3 (Dearing).
have a high endemic infection rate in many mammals, such as
primates, cattle, cats, dogs, rodents and swine. These viruses
are common in raw water sources and are often found along with
other animal viruses. In humans, the viruses cause only
asymptomatic or mild respiratory infections. However, research
suggests that reoviruses may be associated with potentially more
severe illnesses. Reoviruses have been linked to neonatal
hepatitis, extrahepatic biliary atresia, meningitis and
myocarditis. Also, immunocompromised, young and elderly
individuals may become susceptible to severe bacterial
respiratory disease due to an initial reovirus infection.
Due to their
widespread occurrence and the ability of these viruses to
survive a long period of time in the environment, contamination
of water sources has been frequently reported. Animals are
especially prone to infection by these viruses.
Xenotransplantation of animal organs is severely endangered by
potential contamination with these viruses.
reovirus infection by nonmolecular means is very difficult and
is usually based on virus isolation on cell cultures and
electron microscopy. These methods are not very sensitive (Muscillo
et al., 2001) and are likely underestimate the presence of these
viruses in animals and humans. Molecular detection by PCR is the
most sensitive, rapid and specific method for identifying
Help confirm the disease causing agent
Help ensure that animal groups and populations are free of
Early prevention of spread of reoviruses among a
Minimize human exposure to reoviruses
Safety monitoring of biological products and vaccines
that derive from susceptible animals
Muscillo M., La Rosa G., Marianelli C., Zaniratti S.,
Capobianchi M.R., Cantiani L. and Carducci A. (2001) A new
RT-PCR method for the identification of reoviruses in seawater
samples, Water Res. 35:548–556.
Tracheal swab, nasal swab or rectal swab, or 0.2 ml feces, or 0.2 ml whole blood in EDTA (purple top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real time PCR