Zoologix performs primate infectious disease tests by PCR for...


African green monkey endogenous virus


B virus


Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi



Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis



Cronobacter sakazakii


Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian


E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species


Epstein-Barr virus


Gibbon ape leukemia


Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza type A


Lawsonia intracellularis



Lyme disease







Neisseria gonorhoeae

Neisseria meningitidis



Plasmodium species

Reovirus screen

Rhesus rhadinovirus



Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes





Toxoplasma gondii



Trypanosoma cruzi



Valley fever

West Nile virus (WNV)


Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements

Herpes simplex 2 PCR test for primates
primate assay data sheet

Herpes simplex virus type 2 (HSV-2)

Test code:
S0030 - Ultrasensitive qualitative detection of Herpes Simplex Virus Type 2 (HSV-2) by real time polymerase chain reaction


Herpesviruses have been isolated from a wide variety of mammalian and non-mammalian species. The eight human herpesviruses, herpes simplex virus type 1 and 2 (HSV-1 and -2), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), human cytomegalovirus (CMV), and human herpesviruses-6, -7 and -8 (HHV-6, -7 and -8), represent a significant public health problem worldwide. These viruses have been further classified as members of the alpha-herpesvirus (HSV-1, HSV-2 and VZV), the beta-herpesvirus (CMV, HHV-6 and HHV-7) and the gamma-herpesvirus subgroups (EBV and HHV-8). The virus-host relationship is characterized by the benign nature of HSV infection in its usual host, man, and by the fatal disease it causes in accidental hosts, such as the owl monkey or gibbon. Fatal infections caused by HSV have been documented in gibbons, patas and colobus monkeys. A natural epizootic model of Herpesvirus hominis is splenectomized gibbons, which clinically mimic the disease as it occurs in man.

A recent serological study to screen for these herpesviruses in gibbons using antibodies for herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), Epstein-Barr virus (EBV) and cytomegalovirus (CMV) had shown that the prevalence of IgG antibodies against HSV-1, HSV-2, EBV and CMV was 28.2%, 28.2%, 14.1% and 17.9%, respectively. Although cross-reactivities with other monkey herpesviruses cannot be ruled out, the possibility of direct transmission of these human herpesviruses exists. For example, hand rearing of gibbon infants by zoo personnel would provide ample opportunity for human herpesvirus transmission to gibbons. Moreover, the exclusive HSV serotypes within species suggests that these viral infections may have originated from a common source within a species, with subsequent spread from animal to animal, rather than from isolated human-to-gibbon transmissions. Accordingly, HSV-2 infections have been observed to spread from chimpanzee to chimpanzee.

Although virus isolation can be used to diagnose HSV-2 virus infection and determine carrier status, it is not very sensitive or specific, and viral culture increases the potential risk of laboratorians contacting this virus. Serological testing for HSV-2 is difficult because of the close antigenic relation among different herpesviruses.

HSV-2 detection by PCR is the most rapid, sensitive and specific method for diagnosis of this infection and confirmation of carrier status.


  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of HSV-2
  • Early prevention of spread of this virus among a colony
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from primates

Specimen requirement: Lesion swab, or conjunctival swab, or 0.2 ml whole blood in EDTA (purple top) tube.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies: Qualitative real time PCR

Normal range: Nondetected

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