primate assay data sheet
Streptococcus
pyogenes
Test code:
B0077
- Ultrasensitive qualitative detection of Streptococcus pyogenes by
real time
polymerase chain reaction.
Streptococcus pyogenes is a gram-positive
spherical-shaped bacterium. It is beta-hemolytic and belongs to
Lancefield serogroup A, also known as group A streptococci
(GAS). The bacteria
can cause a wide variety of diseases in humans such as acute
pharyngitis, accounting for 15%-30% of cases in children and
5%-10% of cases in adults. During the winter and
spring in temperate climates, up to 20% of asymptomatic
school-aged children may be GAS carriers.
Although GAS usually causes
pharyngitis or impetigo, it can infrequently cause invasive
diseases such as cellulitis, bacteremia, necrotizing fasciitis
and toxic shock syndrome (TSS). Along with Staphylococcus
aureus, GAS is one of the most common pathogens responsible for
cellulitis.
GAS
is ubiquitous in nature and many animal species are carriers of
these bacteria. Close contact between animals and humans can
spread this infection in both directions.
Although culture may be used to detect these bacteria, molecular
detection by PCR is a rapid, highly sensitive and specific
method (Bexiga et al., 2011).
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm a clinical diagnosis of
S. pyogenes infection.
-
Help ensure that colonies are free of S. pyogenes
-
Early prevention of spread of this bacterium
-
Minimize personnel exposure to this bacterium
-
Safety monitoring of biological products that derive from
primates
Specimen requirements:
0.2 ml whole blood
in EDTA (purple top) tube, or serum, or
bacterial culture; or nasal, pharyngeal or oral swabs.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
more information.
Turnaround time: 2 business days
Methodology:
Qualitative real time PCR
Normal range: Nondetected