primate genetic test data sheet
Genotyping of cynomolgus macaque DNA by PCR fragment analysis
- This assay identifies genetic origin of a cynomolgus macaque sample by
microsatellite marker analysis. It uses five
Macaca fascicularis genomic markers to determine the relatedness of
two animals, or to attribute a sample to a source animal.
Cynomolgus macaques (Macaca
fascicularis) are valuable animal models for linkage studies
of genetic diseases. Colony management of these nonhuman
primates therefore requires careful characterization of the
genetic relationships among them. Microsatellite markers are
often used to assist in colony management, conservation work,
and paternity testing of nonhuman primates (Raveendrana et al.,
Microsatellites are short tandem nucleotide repeats (STR) of 2-6
nucleotides, most commonly a repeating CA pattern, usually
occurring in non-coding parts of the genome. They are abundantly
present in the genomes of almost all organisms, and are highly variable in number of
repeats. Since these markers are inherited in Mendelian fashion,
they are especially suitable for linkage analysis because of the
high degree of polymorphism among individual animals.
Identify paternity relationship between two cynomolgus macaques
Linkage analysis for inherited disease management
Colony management of inbreeding
Raveendrana, M., Harrisb, R.A., Milosavljevicb, A., Johnsona, Z.,
Shelledya, W., Cameronc, J., Rogers, J. (2006) Designing new
microsatellite markers for linkage and population genetic
analyses in rhesus macaques and other nonhuman primates.
0.2 ml whole blood in EDTA (purple top) tube, or buccal swab, or 0.2 ml fresh
or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for more information.
5 business days
PCR / microsatellite marker analysis