rodent and rabbit assay data sheet
B0046 - Ultrasensitive qualitative detection of
bacteria by real time PCR.
is an important cause of morbidity and mortality in some common
domestic animals, including dogs, pigs and rabbits. Infection of
horses, seals, cats and humans have also been reported. In dogs,
infectious tracheobronchitis is caused by
canine adenovirus-2, and canine parainfluenza virus, or by co
infections by combinations of these organisms. Infection can
result in acute or chronic cough, ciliary impairment, local
respiratory immunosuppression, and predisposition to secondary
lower-respiratory infections. In animal shelters, "kennel cough"
in dogs represents a major management problem; it is readily
transmissible, reduces adoption rates for affected animals, and
requires intensive medical management. Dogs with infectious
tracheobronchitis are euthanized in some shelters.
Tracheobronchitis, conjunctivitis and rhinitis
(upper-respiratory tract disease), mandibular lymphadenopathy,
and pneumonia have been reported in cats infected with
Positive identification of
B. bronchiseptica in cats is absolutely required to
institute the appropriate treatment because upper-respiratory
infection (URI) in cats can also be caused by herpesvirus,
calicivirus, Mycoplasma species, and Chlamydia psittaci
infection. It is believed that many cats could be carriers of
the bacteria because many B.
bronchiseptica culture-positive cats are clinically
normal. When these cats are co-infected with other pathogens,
URI will develop. Although it is not known how likely cats are
to infect rabbits with B.
bronchiseptica, putative cross-species transmission
of B. bronchiseptica
has been described.
detection of B. bronchiseptica
is very important for surveillance of the disease and control of
the bacterial infection. Early detection can be crucial to
successful treatment of the disease with antibiotics and to
control the spread of the disease. The conventional method of
detection, by culture, is specific but lacks sensitivity. It
also takes 3 to 7 days to obtain a result. Current serological
tests do not differentiate the closely related
Only molecular detection by PCR can give both rapid and specific
identification of B.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of B.
Help ensure that rabbit groups are free of
Early prevention of spread of
Minimize personnel exposure to
Safety monitoring of biological products that derive
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time polymerase chain reaction