Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mouse minute virus and mouse parvovirus PCR tests

rodent and rabbit assay data sheet

Mouse minute virus (MMV) and mouse parvovirus (MPV)

Test code: S0098 - Ultrasensitive qualitative detection but not differentiation of mouse minute virus and all well-described mouse parvovirus serotypes (MPV types 1, 2, 3, 4 and 5) by real time PCR

S0098 is included on P0023 Mouse Fecal Panel, on P0032 Mouse Minipanel and on P0029 - Mouse Essentials Panel


Mouse minute virus (MMV) (formerly minute virus of mice) and mouse parvovirus (MPV) are among the most prevalent infectious agents found in laboratory mouse colonies. Although the immunosuppressive strain of MMV (MMVi) can induce a potentially lethal renal hemorrhagic disease when experimentally inoculated into neonatal mice, no significant clinical symptoms have been reported for mice naturally infected with MMV. Similarly, no significant adverse effects have been observed in mice naturally or experimentally infected with MPV. Nevertheless, infection with MMV or MPV can have significant deleterious effects on research due to their immunomodulatory effects both in vivo and in vitro. In addition, there is significant potential for MMV and MPV to be transmitted among animals within a facility due to the high degree of environmental stability of these agents. Thus, identification of infected laboratory mice is critical to minimize the impact of these viruses on research.

Mice are infected primarily by direct contact with virus shed in feces or urine. Parvoviruses are capable of surviving in the environment for an extended period of time (weeks). Thus fomite transmission is likely.

Serology testing for these viruses has been used extensively to test for the immune status of mice. However, this testing approach is often inadequate to control the spread of the viruses, largely because it takes close to 2 weeks to generate a detectable serological response when the mice are exposed to these viruses. Secondly, the sensitivity of serological testing is often not comparable to molecular detection by PCR. Thirdly, serological testing cannot be used for immunosuppressed mice, such as nude mice. Fourthly, fecal or urine samples can be conveniently used in the PCR technique to detect the presence of virus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of mouse minute virus or mouse parvovirus
  • Help ensure that vivariums are free of these viruses
  • Early prevention of spread of these viruses among a vivarium
  • Minimize personnel exposure to these viruses
  • Safety monitoring of biological products that derive from mice

Specimen requirements: Fecal pellet, or 0.2 ml urine, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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