rodent and rabbit assay data sheet
Pneumonia virus of mice (PVM)
Ultrasensitive qualitative detection of pneumonia virus of mice
by reverse transcription coupled real time PCR
S0139 is included on
P0029 - Mouse Essentials Panel
of mice (PVM) belongs to the family Paramyxoviridae, subfamily
Other members of Pneumovirus include human and bovine syncytial
viruses (HRSV and BRSV, respectively).
laboratory rodents by PVM is widespread because clinical signs
of disease and gross lesions are absent in infections of
immunocompetent rodents, making carriers difficult to detect.
(Cook et al., 1998).
PVM infection of immunodeficient rodents, such as nude mice, may
cause a persistent wasting disease syndrome.
detection can identify rodents previously exposed to PVM, but
serology is not a good tool to identify current carriers of this
virus. Molecular detection by PCR is the most sensitive,
specific and rapid means to identify these carriers.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of PVM
Help ensure that vivariums are free of PVM
Early prevention of spread of this virus among a
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
Cook, P.M., Eglin, R.P. and Easton, A.J. (1998) Pathogenesis of
pneumovirus infections in mice: detection of pneumonia virus of
mice and human respiratory syncytial virus mRNA in lungs of
infected mice by in situ
hybridization. J. Gen. Virol. 79: 2411-2417.
0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
or 0.2 ml cell culture.
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
reverse transcription coupled real time polymerase chain
Normal range: Nondetected