rodent and rabbit assay data sheet
Lactate dehydrogenase-elevating virus (LDV)
Test code: S0137
- Ultrasensitive qualitative detection of Lactate dehydrogenase
elevating virus by reverse transcription coupled real time PCR
S0137 is included on P0029 -
Mouse Essentials Panel
dehydrogenase-elevating virus (LDV) belongs to the
Togaviridae family and
is included in the genus
Arterivirus. It is also known as Lactate Dehydrogenase
Virus. LDV is a
mouse-restricted virus that
causes a lifelong persistent infection in the blood.
Mouse infections result in alterations in a number of
physiological functions such as immunological responses and
enzyme levels. Such
changes can severely affect experimental results.
the virus between mice in a colony is rare.
The most frequent route of transmission is passage of
contaminated stocks of tumor cells or other biological materials
through the mice. It
is difficult to identify infected mice because they do not show
gross clinical symptoms.
the virus by serology testing is unreliable and culture of the
virus is difficult (Brinton, 1986).
Since infected mice show elevated levels of lactate
dehydrogenase, detection has relied in the past on indirect
determination by enzyme level.
However, hemolysis, tissue damage and freezing of plasma
samples can also result in non-specific elevation of this enzyme
(Brinton, 1986), so false positive results are common by this
method. As LDV
persistently circulates in the blood during infection, detection
of viral RNA by reverse transcription coupled PCR represents a
sensitive, specific and rapid method of detection of this virus
Help confirm the disease causing
Shorten the time required to confirm a clinical
diagnosis of LDV
Help ensure that vivariums are free of LDV
Early prevention of spread of LDV among a vivarium
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
(1986) Lactate dehydrogenase-elevating virus, p. 389-420. In
P.N. Bhatt, R.O. Jacoby, H.C. Morse
III, and A.E. New (ed.), Viral and mycoplasmal
infection of laboratory rodents: effects on biomedical research.
Academic Press, Inc., Orlando, Fla.
0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube,
or 0.2 ml cell culture
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
for more information.
2 business days
reverse transcription coupled real time polymerase chain
Normal range: Nondetected