Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Lactate dehydrogenase elevating virus PCR test

rodent and rabbit assay data sheet

Lactate dehydrogenase-elevating virus (LDV)

Test code: S0137 - Ultrasensitive qualitative detection of Lactate dehydrogenase elevating virus by reverse transcription coupled real time PCR

S0137 is included on P0029 - Mouse Essentials Panel

Lactate dehydrogenase-elevating virus (LDV) belongs to the Togaviridae family and is included in the genus Arterivirus. It is also known as Lactate Dehydrogenase Virus.  LDV is a mouse-restricted virus that  causes a lifelong persistent infection in the blood.  Mouse infections result in alterations in a number of physiological functions such as immunological responses and enzyme levels.  Such changes can severely affect experimental results.

Transmission of the virus between mice in a colony is rare.  The most frequent route of transmission is passage of contaminated stocks of tumor cells or other biological materials through the mice.  It is difficult to identify infected mice because they do not show gross clinical symptoms.

Detection of the virus by serology testing is unreliable and culture of the virus is difficult (Brinton, 1986).  Since infected mice show elevated levels of lactate dehydrogenase, detection has relied in the past on indirect determination by enzyme level.  However, hemolysis, tissue damage and freezing of plasma samples can also result in non-specific elevation of this enzyme (Brinton, 1986), so false positive results are common by this method.  As LDV persistently circulates in the blood during infection, detection of viral RNA by reverse transcription coupled PCR represents a sensitive, specific and rapid method of detection of this virus in mice.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of LDV
  • Help ensure that vivariums are free of LDV
  • Early prevention of spread of LDV among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Brinton, M. (1986) Lactate dehydrogenase-elevating virus, p. 389-420. In P.N. Bhatt, R.O. Jacoby, H.C. Morse III, and A.E. New (ed.), Viral and mycoplasmal infection of laboratory rodents: effects on biomedical research. Academic Press, Inc., Orlando, Fla.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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