Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Reovirus 3 PCR test for rodents
rodent and rabbit assay data sheet

Reovirus type 3 (REO3)

Test code: S0101 - Ultrasensitive qualitative detection of reovirus type 3 by reverse transcription coupled real time PCR

 

Reovirus type 3 (Reo-3) can infect many different rodent species and is prevalent in laboratory rodent colonies. It is a common contaminant of transplantable tumor cell lines and other biological materials.

Infection of neonatal mice with Reo-3 can lead to a multi-systematic disease that is characterized by necrotizing hepatitis, myocarditis, pancreatitis, and meningoencephalitis. Steatorrhoea often develops secondary to liver disease and results in “oily skin” appearance.

Reo-3 virus can be transmitted between animals by aerosol and fecal–oral routes, and fomite transmission is possible because the virus is environmentally stable. One infected animal can easily lead to rapid outbreak of the disease and therefore rapid diagnosis is essential to prevent transmission through an animal facility.

Several methods are currently employed to detect Reo-3 infection in rodents and contaminated biological materials. Serological detection of anti-viral antibodies has typically been used to diagnose Reo-3 infection in rodents. However, serological assays cannot detect virus infections directly in immunodeficient strains of rodents that do not generate a humoral immune response, and even in immunocompetent rodents the time needed for seroconversion is too long for rapid diagnosis of the disease.

Molecular detection by real time PCR, on the other hand, has proven to be a useful technique for detection of Reo-3 (Steele, 1995), as it is highly sensitive and also very specific. Unlike serology testing, there is no cross-reactivity with other similar viruses. Real time PCR is also an attractive alternative to the rodent antibody production (RAP) test for detection of Reo-3 contamination in biological materials (Compton & Riley 2001). Compared to RAP testing, real time PCR offers faster turnaround time and lower cost.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Reo-3
  • Help ensure that vivariums are free of Reo-3
  • Early prevention of spread of this virus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Compton, S.R. and Riley, L.K. (2001) Detection of infectious agents in laboratory rodents: traditional and molecular techniques. Comparative Medicine 51:113–119.
Steele, M.I. (1995) Reovirus 3 not detected by reverse transcriptase-mediated polymerase chain reaction analysis of preserved tissue from infants with cholestatic liver disease. Hepatology 21: 697–702.

Specimen requirements: Fecal pellet, or nasal or tracheal swab, or 0.2 ml fresh, frozen or fixed tissue, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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