Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Hantavirus PCR test

rodent and rabbit assay data sheet

Hantavirus

Test code: S0135 - Ultrasensitive qualitative detection of Hantavirus by reverse transcription coupled real time PCR. This test does not detect Seoul virus - see test code S0224 for Seoul virus detection.

 S0135 is included on P0029 - Mouse Essentials Panel

Hantaviruses are a group of viruses that are transmitted by rodents through urine, droppings, and saliva. One of them, Sin Nombre virus, is found in deer mice in North America. Sin Nombre virus is the cause of Hantavirus Pulmonary Syndrome (HPS) in humans.

Hantaviruses belong to the Bunyaviridae family of viruses. There are five genera in this family: Orthobunyavirus, Nairovirus, Phlebovirus, Tospovirus, and Hantavirus. Like all members of this family, hantaviruses have a genome comprised of three negative-sense single-stranded RNA segments, and so are classified as negative sense RNA viruses.

Inhalation of dust contaminated by rodent excreta or saliva can result in transmission of these viruses to humans. Infected people will develop HPS one to six weeks after inhaling the virus. The disease begins with 2-6 days of flu-like illness including fever, muscle soreness, headache, nausea, vomiting and fatigue. As the disease progresses it causes shortness of breath due to fluid filled lungs; hospital care is then required. It is usually a serious infection and about 1 out of 3 infected patients have died.

The deer mouse (Peromyscus maniculatus) is the main carrier of hantaviruses in the western United States but other wild rodents may also be potential carriers. The deer mouse can carry and shed the virus without showing any clinical signs. In one study, it was shown that about 14% of over 1,100 deer mice in Washington State had been infected with Sin Nombre virus.

HPS is not known to be transmitted from one person to another, nor to be transmitted by farm animals, dogs, cats or rodents purchased from pet stores.

Serological detection of these viruses is not effective in identifying rodents that are shedding virus because many wild-caught rodents may have had past exposure and thus antibodies to the viruses. Studies have shown that even if infected animals have not mounted a detectable antibody response, the virus may be detected by PCR. When infected animals progress to a chronic carrier state, antibody level may remain detectable but does not correlate with the shedding of virus. PCR detection of the virus in blood, saliva, urine or feces can confirm shedding of viruses by infected animals (Kuenzi et al., 2005).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of hantavirus
  • Help ensure that rodent populations and colonies are free of hantavirus
  • Early prevention of spread of hantavirus among a population or in a geographic area
  • Minimize human exposure to hantavirus
  • Safety monitoring of biological products that derive from rodents

References:
Kuenzi, A.J., Douglass, R.J., Bond, C.W., Calisher, C.H. and Mills, J.N. (2005) Long-term dynamics of Sin Nombre viral RNA and antibody in deer mice in Montana. J. Wildl. Dis. 41:473-481.

Specimen requirements: 1 fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or environmental wipes/swabs, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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