avian & livestock assay data sheet
- Ultrasensitive detection of bovine adenovirus by real time
Bovine adenovirus is a double-stranded DNA virus belonging to
that causes disease in cattle.
Infected animals may develop diarrhea, reduced appetite and
abdominal distension. Respiratory symptoms may also develop,
including coughing, nasal discharge, dyspnea and tachypnea.
Infected animals may get even sicker if a secondary infection
occurs. Classic signs of generalized disease, such as fever,
weight loss, depression, weakness, enlarged lymph nodes and
lethargy are often seen. Death can result.
Infected cattle can continually shed virus for approximately 10
days in respiratory secretions and feces.
In some individuals persistent infection may result and
shedding of the virus may continue indefinitely.
Diagnosis of the infection can be by viral culture or
serological testing. However, viral culture diagnosis of this
infection is not sensitive and serological testing requires
paired testing of serum samples, which requires long lead times.
Molecular detection by polymerase chain reaction (PCR) is a
highly specific, sensitive and rapid detection method (Sibley et
Help confirm the disease causing agent
Identify bovine adenovirus carriers
Screen research materials for the presence of bovine
Help ensure that animal groups are free of bovine adenovirus
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
Sibley, S.D., Goldberg, T.L. and Pedersen, J.A. (2011)
Detection of known and novel adenoviruses in
cattle wastes via broad-spectrum primers. Appl. Environ.
0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top), or 0.2 ml cell culture, or 0.2 ml feces, or 0.2 ml
urine, or 2 respiratory or nasal swabs, or 0.2 ml fresh, frozen
or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
for more information.
Turnaround time: 2 business days
Methodology: Qualitative real time PCR
Normal range: Nondetected