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avian
& livestock assay data sheet
Porcine circovirus type 1
Test code:
S0187
- Ultrasensitive detection of porcine circovirus type 1 by real time PCR
Porcine circoviruses (PCVs)
are single-stranded nonenveloped DNA viruses containing an
unsegmented circular genome protected by an icosahedral viral
capsid. The whole virus is approximately 17nm in diameter.
PCVs belong to the family
circoviridae and are considered the smallest viruses replicating
autonomously in the nucleus of infected eukaryotic cells; they
use the host polymerase for genome amplification. Two strains of
porcine circovirus have been identified, type 1 and type 2.
PCV type 1 was first
identified in 1974 and is not known to cause disease in swine,
although
there is circumstantial evidence that PCV1 may be
involved in congenital tremors.
PCV
type 1 recently garnered media attention because the virus was
discovered in some human vaccines (Baylis et al., 2011), raising
concerns about contamination in human vaccine production.
Diagnosis of PCV type 1
infection usually relies on molecular detection (eg polymerase
chain reaction), which gives high sensitivity and specificity.
Utilities:
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Help confirm the disease causing agent
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Help identify PCV1 carriers
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Help ensure that herds and populations are free of
PCV1
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Early prevention of spread of the virus among animals
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Minimize human exposure to the virus
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Safety monitoring of biological products that derive
from animals
References:
Baylis, S.A.,
Finsterbusch, T., Bannert, N., Blümel, J. and Mankertz, A.
(2011)
Analysis of porcine circovirus type 1 detected in Rotarix
vaccine. Vaccine29:690-697.
Specimen requirements:
0.2 ml
whole blood in EDTA (purple top) tube, or 0.2 ml
saliva, or oral swab, or 0.2 ml fresh, frozen or fixed
tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time PCR
Normal range:
Nondetected
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