avian & livestock assay data sheet
Test code: B0100
- Ultrasensitive qualitative detection of
Brachyspira pilosicoli by real time polymerase chain reaction.
There are at least five distinct species of
known to infect the large intestine of swine, namely
and Serpulina murdochii.
hyodysenteriae, which causes swine dysentery, and
which causes intestinal spirochetosis, are pathogenic.
Infection of pigs with B.
pilosicoli results in non-fatal intestinal spirochetosis.
Weaned pigs, primarily 8-16 weeks of age, are especially
susceptible to infection. Uncomplicated intestinal spirochetosis
is usually not associated with mortality, but economic loss can
be severe due to reduced growth rate of the pigs. The major
clinical symptom is diarrhea. Feces from infected pigs are
usually soft and wet with a consistency like "wet cement". In
later stage of disease progression, feces may change to a watery
consistency with a small amount of mucus (i.e., have an "oily"
sheen). During recovery or in chronic cases, feces may contain
thick tags of mucus. In rare circumstances, blood may be seen in
feces. Affected pigs generally remain alert and active, but
appetite is decreased. Infected pigs may show abdominal
discomfort and/or may appear gaunt and develop rough hair coats.
In addition to swine, B. pilosicoli
infects humans, nonhuman primates, dogs, and several species of
birds. Humans infected with these bacteria may develop mild
nausea, abdominal discomfort, and severe headaches.
All five of these distinct species of
Brachyspira are morphologically indistinguishable; thus, enteric
spirochetal diseases in swine can rarely be confirmed by
histopathologic examination alone. However, this pathogen can be
specifically identified by polymerase chain reaction (Fellstrom
et al., 1997; Park et al., 1995).
Help confirm the disease causing agent
Help ensure that swine herds are free of
Brachyspira pilosicoli bacteria
Early prevention of spread of the bacteria among a herd
Minimize human exposure to the bacteria
Fellstrom, C., Pettersson, B., Thomson, J., Gunnarsson, A., Persson, M.
and Johansson, K.E. (1997) Identification of Serpulina
species associated with porcine colitis by biochemical analysis
and PCR. J. Clin. Microbiol. 35:462-467.
Park, N.Y., Chung, C.Y., McLaren, A.J., Atyeo, R.F. and Hampson, D.J.
(1995) Polymerase chain reaction for identification of human and
porcine spirochaetes recovered from cases of intestinal
spirochaetosis. FEMS Microbiol. Letters. 125:225-230.
0.2 ml feces, or rectal swab, or environmental swab, or 0.2 ml whole blood
in EDTA (purple top) tube, or 0.2 ml serum.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR