& livestock assay data sheet
Porcine epidemic diarrhea virus (PEDV)
- Ultrasensitive detection of porcine epidemic diarrhea virus by reverse
transcription coupled real time PCR
Porcine epidemic diarrhea virus (PEDV) belongs to the family
contains an enveloped, single-stranded positive-sense RNA
genome. This virus infects the cells lining the pig’s small
intestine, causing porcine epidemic diarrhea, a condition of
severe diarrhea and dehydration. Although older hogs may become
ill and lose weight, they usually recover from the infection.
However, infected newborn piglets usually die within five days
of contracting the virus. Fortunately, PEDV cannot be
transmitted to humans.
Infected pigs can shed a huge number of viral particles in their feces,
and the virus survives well in the environment. For these
reasons, this virus has been detected in samples collected from
pig collection points, slaughter facilities, transportation
vehicles and many different fomites. Transmission can be through
inhalation or direct contact with the infected environment, but
most often occurs through the fecal-oral route. PEDV survives in
the environment much better in colder temperatures; thus,
disease outbreaks occur more frequently in winter.
Culture detection of this virus is not a method of choice because the
virus does not grow well in culture. Antigen detection of the
virus in feces has a low sensitivity. However, molecular
detection by polymerase chain reaction is rapid, sensitive and
specific, and is a good alternative to those traditional methods
(Zhao et al., 2014).
Help confirm the disease causing agent
Identify PEDV virus carriers
Help ensure that facilities and groups of pigs are free of
Early prevention of spread of this virus
Minimize human exposure to this virus
Safety monitoring of biological products that derive
Zhao, P.D., Bai, J., Jiang, P., Tang, T.S., Li, Y., Tan, C. and Shi, X.
(2014) Development of a multiplex TaqMan probe-based real-time
PCR for discrimination of variant and classical porcine epidemic
diarrhea virus. J. Virol. Methods 206:150-155.
whole blood in EDTA (purple top) tube, or 0.2 ml feces, or
fecal or rectal swabs, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
Qualitative reverse transcription coupled real time PCR