avian & livestock assay data sheet
- Ultrasensitive screen for
Cryptosporidium by real time polymerase chain reaction.
This screen detects but does not differentiate
species, utilizing a real time PCR procedure to achieve highly
Test P0008 is
- waterborne pathogens screening panel
the 13+ species in the
Cryptosporidium genus have been confirmed as
causative agents of human disease.
Cryptosporidium is a
parasitic protozoan that is transmitted by multiple routes; the
animal host range is diverse. The following
species are currently accepted, on the basis of host
specificity, pathogenesis, morphology and genotyping:
C. parvum, C. wrairi, C. felis, C. canis, C. andersoni,
C. muris and C. ubiquitum
birds: C. baileyi, C.
reptiles: C. serpentis
fish: C. molnari
analyses have been largely based on sequencing of the small
subunit rRNA gene (18S rRNA), the hsp 70 gene, or other
housekeeping or structural genes. These analyses reveal that the
species interact in complex ways with hosts. For example, the
specific host of C. felis
is cats, but this species has also been isolated from a cow,
while C. andersoni
is morphologically close to C. muris but infects cattle rather than mice. And
C. parvum includes a
complex of subspecies that specifically infect cattle, pigs,
kangaroos, ferrets or monkeys.
advance of molecular techniques, knowledge of the epidemiology
of human cryptosporidiosis has significantly increased. It has
been shown that the vast majority of human cases are caused by
(synonymous with C. parvum
genotype 1) and C. parvum
(synonymous with C. parvum
genotype 2). Other species, including
C. felis, C. canis
and C. muris can
also infect humans and are linked to clinical disease, not only
in immunocompromised patients but also in immunocompetent
PCR is a rapid and
extremely sensitive technique for detection of
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
Help ensure that bird flocks are free of
Early prevention of spread of this protozoan
Minimize human exposure to this protozoan
Thomas, A.L. and Chalmers, R.M. (2003) Investigation of the
range of Cryptosporidium
species detected by commercially available antibody-based tests.
Proceedings of the Health Protection Agency Inaugural
Conference, Warwick, September.
Rectal swab, or cloacal swab, or 0.2 ml feces.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
Turnaround time: 2
Qualitative real time PCR