avian & livestock assay data sheet
Pigeon circovirus (PiCV)
detection of pigeon circovirus by real time polymerase chain reaction
circovirus (PiCV) infections have been described in several
regions of the world. Infected pigeons may develop symptoms such
as lethargy, anorexia, weight loss, reduced race performance,
respiratory distress and diarrhea.
genome of PiCV (also known as columbid circovirus or CoCV) has
been found to share low-level DNA homology with psittacine beak
and feather disease virus (BFDV). The virus currently is
classified as a tentative member of family Circoviridae and the
genome of PiCV is similar to other members of the family: It is
a circular, single-stranded DNA of approximately 1.76–2.04 kb in
size, contained within a non-enveloped protein coat.
culture identification of virus has not been described.
Histopathological examination of infected young birds reveals
the presence of characteristic inclusions in lymphoid tissue
such as the bursa of Fabricius. As this organ shrinks in size as
birds grow older and is tiny or absent in adult birds, diagnosis
by histopathology alone is therefore unreliable. Furthermore,
this method does not allow early diagnosis of infection.
detection by PCR is very useful because it can identify the
presence or absence of the viral genome in blood (Hattermann et
al, 2002) and a number of different tissues (Todd et al, 2002),
and can assist the post mortem diagnosis of PiCV disease. PCR
detection of PiCV is highly sensitive and specific.
Help confirm the disease causing agent
Help ensure that bird populations are free of PiCV
Early prevention of spread of the virus among bird
Minimize human exposure to the virus
Hattermann, K., Soike, D., Grund, C. and Mankertz A, (2002) A
method to diagnose Pigeon circovirus infection in vivo. J. Virol.
Todd, D., Duchatel, J.P., Weston, J.H.,
Ball, N.W., Borghmans, B.J., Moffett, D.A. and Smyth, J.A.
(2002) Evaluation of polymerase chain reaction and dot blot
hybridization tests in the diagnosis of pigeon circovirus
infections. Vet. Microbiol. 89:1-16.
0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml fresh, frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR