avian & livestock assay data sheet
Porcine circovirus 2 (PCV2)
detection of porcine circovirus type 2 by real time PCR
circoviruses (PCVs) are small, non-enveloped viruses
with a single-stranded, closed-circular
DNA. They belong to the genus
Circovirus of the family Circoviridae. Two
are known, PCV1 and PCV2.
circovirus type 1 (PCV1) can be detected in swine populations,
but unlike PCV2 it is not associated with any clinical disease.
the major cause of postweaning multisystemic wasting syndrome
(PMWS). It is also found in association with numerous other
conditions. These conditions include porcine dermatitis and
nephropathy syndrome (PDNS), porcine respiratory disease complex
(PRDC), congenital tremors (CT) type AII, reproductive failure
be detected in virtually every swine herd. The most common
presentation of PCV2-associated disease is pneumonia. However,
the underlying cause of PCV2-associated disease may be other
pathogenic infections, such as porcine parvovirus (PPV) or
porcine reproductive and respiratory syndrome virus (PRRSV).
Thus, differentiating PCV2 infection from other viral infections
is important in order to design an effective treatment plan.
infected with PCV2 may develop no symptoms, but they can carry
and shed the virus (Larochelle et al., 2000). Serology testing
is insufficient to identify these carriers, since many pigs in a
herd may have been exposed to the virus. Molecular detection by
polymerase chain reaction (PCR) is a rapid, sensitive and
specific method for identifying these infected pigs.
In addition, PCV1 and PCV2 are antigenically very similar
and differentiation of the two viruses by serological testing is
difficult. These two viruses, however, can be differentiated by
Help confirm the disease causing agent
Identify PCV2 carriers
Help ensure that animal colonies and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
Larochelle, R., Bielanski, A., Müller, P. and Magar,
R. (2000) PCR
Detection and Evidence of Shedding of Porcine Circovirus Type 2
in Boar Semen. J. Clin. Microbiol. 38: 4629–4632.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml saliva, or oral swab, or 0.2 ml fresh, frozen or
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR