avian & livestock assay data sheet
Bovine viral diarrhea virus (BVDV)
qualitative detection of bovine viral diarrhea (BVD) virus by
reverse transcription coupled real time PCR
diarrhea virus (BVDV) is a single-stranded positive-sense RNA
virus classified as a pestivirus belonging to the Flaviviridae
family. Infection of cattle with this disease can pose severe
economic losses to the dairy and beef industries, primarily due
to decreased reproductive performance. BVDV occurs nearly
Two major BVDV
strains have been identified, designated as genotype 1 or
genotype 2 on the basis of antigenic variation and differences
in sequences in the 5' UTR of the viral genome. Infection by
different strains of BVDV can have different clinical symptoms.
Infected cattle may develop gastrointestinal disease,
immunosuppression with secondary infections, thrombocytopenia
and reproductive failure. In infected pregnant cows, the virus
is transmitted to a fetus with a high degree of efficiency,
leading to fetal loss, malformations or birth of a persistently
with BVDV may result in the birth of a calf immunotolerant to
BVDV, with an inapparent persistent infection. These calves can
become carriers and remain viremic throughout their
lifetimes, shedding infectious BVDV and acting as reservoirs
of the virus in cattle herds.
testing is not effective for identifying viremic, immunotolerant
testing is also not very useful for testing pooled serum samples
because of its low sensitivity. On the other hand, molecular
detection of the virus by PCR is a rapid, specific, sensitive
and cost effective method for identifying viremic individuals (Weinstock,
et al., 2001).
Help confirm the disease causing agent
Identify BVD carriers
Help ensure that animal herds and populations are free of BVD
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
0.2 ml feces,
or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml serum, or 0.2 ml tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real time PCR