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avian & livestock assay data sheet
Staphylococcus xylosus
Test
code:
B0094 - Ultrasensitive qualitative detection of Staphylococcus
xylosus
bacteria by real time polymerase chain reaction
Members of the
Staphylococcus genus are gram-positive
bacteria that form clusters of cells. Like most
staphylococcal species, Staphylococcus
xylosus is coagulase-negative and
non-hemolytic, and is usually found as normal
flora on the skin of humans and animals, and in
the environment.
Although S. xylosus
generally does not cause disease in animals or
humans, it has infrequently been implicated in
some disease cases, such as nasal dermatitis in
gerbils, pyelonephritis and endocarditis in
humans, avian staphylococcosis, and bovine
intermammary infections.
S. xylosus is a source
of proteolytic enzymes commonly used in food
processing. It is essential in making sausage
and some cheeses. Its growth is not affected by
yeasts during the processing of cheese, and its
proteolytic enzymes and lecithinases improve the
flavor and tenderness of sausage.
Traditionally, culture
identification of S. xylosus required
laborious colony isolation and biochemical
analysis because Staphylococcus
bacteria are ubiquitously present in the
environment. Molecular detection by polymerase
chain reaction (PCR) is rapid, sensitive and
specific, and is a useful alternative to the
traditional method (Blaiotta et al., 2003).
Utilities:
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Help confirm the disease causing agent
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Shorten the time required to confirm a clinical
diagnosis of
Staphylococcus xylosus infection.
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Help ensure that flocks are free of
Staphylococcus xylosus
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Early prevention of spread of
Staphylococcus xylosus
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Minimize personnel exposure to these bacteria
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Safety monitoring of biological products and vaccines
that derive from susceptible birds or
mammals
Reference:
Blaiotta, G., Pennacchia, C., Parente,
E. and Villani, F. (2003) Design and evaluation
of specific PCR primers for rapid and reliable
identification of Staphylococcus xylosus strains
isolated from dry fermented sausages. Syst.
Appl. Microbiol. 26:601-610.
Specimen requirements: 0.2 ml
whole blood in eDTA (purple top) tube, or
nsapharyngeal swab, or 0.2 ml culture, or environental swab, or
food swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay
in shipping, or during very warm weather,
refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens
should be shipped so as to remain frozen in
transit. See shipping
instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time PCR
Normal range:
Nondetected
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