avian & livestock assay data sheet
- Ultrasensitive qualitative detection of Wesselsbron virus by
reverse transcription coupled real time polymerase chain reaction
Wesselsbron virus is a flavivirus, an enveloped, positive-sense RNA
virus. The virus is transmitted by mosquitoes in the genus
Aedes including A. caballus
and A. circumluteolus.
Domesticated herbivores are the major reservoirs of this virus.
Infections of sheep, cattle, and goats are often subclinical,
but disease may be severe in sheep with preexisting liver
diseases. Newborn lambs and goat kids are most susceptible, and
mortality may occur. Occasional abortion in ewes is seen,
together with congenital malformation of the central nervous
system with arthrogryposis of the ovine (and also the bovine)
fetus, and hydrops amnii in ewes. Humans can be infected with
the virus but the disease is usually mild and nonfatal.
In the past, serological diagnosis has been based on
hemagglutination-inhibition, complement fixation, and virus
neutralization, but none of these assays are very sensitive or
specific. However, molecular detection by PCR is rapid,
sensitive and specific, and is a good alternative to traditional
methods of detection (Johnson et al., 2012).
Help confirm the disease causing agent
Identify Wesselsbron virus carriers
Help ensure that herds and populations are free of
Early prevention of spread of this virus among animals
Minimize human exposure to this virus
Safety monitoring of biological products that derive from
Johnson, N., Voller, K., Phipps, L. P., Mansfield, K. and Fooks, A. R.
(2012). Rapid molecular detection methods for Arboviruses of
livestock of importance to Northern Europe. J. of Biomed.
Biotech. 2012: 719402.
Specimen requirements: 0.2 ml whole blood
in EDTA (purple top) tube, or 0.2 ml
cerebrospinal fluid, or 0.2 ml fresh or
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative reverse
transcription coupled real time PCR
Normal range: Nondetected
Wesselsbron virus PCR test