We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli

Brucella

Cache Valley virus

Camelpox

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

Ebola Reston

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Brucella PCR test

avian & livestock assay data sheet

Brucella  NOTE: THIS TEST IS NOT PERFORMED ON SAMPLES TAKEN FROM LIVESTOCK OWNED OR LOCATED IN THE STATE OF CALIFORNIA.

Test code: B0075 - Ultrasensitive detection of Brucella bacteria by real time PCR. This assay detects but does not differentiate Brucella abortus, B. microti, B. melitensis, B. pinnipedialis, B. suis, B. canis, B. ovis and B. neotomae.

  

Brucellosis is an infectious disease caused by bacteria of the genus Brucella.  Various Brucella species have been shown to infect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animal species. Humans can become infected when they contact these infected animals or their products.

In humans, brucellosis can cause a range of symptoms similar to influenza, and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous system or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain and fatigue.

Although brucellosis is not common in United States, it frequently occurs in countries that have poor animal disease control programs. Travel to these countries can result in infection with these bacteria. Humans are usually infected by eating or drinking something that is contaminated with the bacteria, by inhaling the bacteria, or by exposure of wounds to these bacteria. Occupational exposure is one of the major routes for human infection. The chronic nature of the disease means that infected people or animals can become carriers of the bacteria.

Diagnosis of brucellosis has routinely been by culture detection of the bacteria, but culture has very low sensitivity and so is less useful for subacute and chronic carriers. Molecular detection by PCR, on the other hand, can provide rapid, sensitive and specific identification of these bacteria in a sample (Mitka et al., 2007).

Utilities:

  • Help confirm the disease causing agent
  • Identify Brucella carriers
  • Help ensure that herds and other animal populations are free of Brucella
  • Early prevention of spread of these bacteria among animals
  • Minimize human exposure to these bacteria
  • Safety monitoring of biological products that derive from susceptible animals

References:
Mitka, S., Anetakis, C., Souliou, E., Diza, E. and Kansouzidou, A. (2007). Evaluation of Different PCR Assays for Early Detection of Acute and Relapsing Brucellosis in Humans in Comparison with Conventional Methods. J. Clin. Microbiol. 45: 1211-1218

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml milk

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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