Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Aspergillus PCR test
environmental, wildlife and zoo assay data sheet

Aspergillus species

Test code: F0009 - Ultrasensitive qualitative detection of most common Aspergillus species by real time PCR

 

Aspergillus is a filamentous fungus that occurs ubiquitously in the environment. It is found in soil, on plants, in decaying organic matter, and in household dust and building materials. Most people inhale Aspergillus spores every day without being affected, but for immunocompromised persons such as AIDS patients and cancer patients, inhaling the fungal spores may cause illness. The spectrum of illness includes allergic reactions, lung infections, and infections in other organs.

There are more than 185 species of Aspergillus; the most common species are Aspergillus fumigatus and Aspergillus flavus. Other common species include Aspergillus terreus, Aspergillus nidulans, and Aspergillus niger.

Aspergillus infections in birds, cattle, horses, pigs, primates and other species have been reported infrequently. Pulmonary symptoms are often seen in these cases. Healthy unstressed animals are generally resistant to even high levels of spores. However, young and old animals, those on antibiotics, and those whose immune systems are suppressed by surgery, reproduction, environmental changes, capture, or shipping may become infected.

Since many animals have had prior exposure to Aspergillus, serological testing is not useful or reliable. Although traditional culture detection of Aspergillus is highly specific, culture of this organism is slow and sensitivity is low. However, molecular detection by polymerase chain reaction (PCR) is both specific and sensitive, and is a rapid and effective alternative to other methods (Buess et al., 2012).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Aspergillus infection.
  • Detection of this fungus in buildings and at worksites
  • Early prevention of the spread of this fungus
  • Minimize personnel exposure to this fungus
  • Safety monitoring of biological products and vaccines

References:
Buess, M., Cathomas, G., Halter, J., Junker, L., Grendelmeier, P., Tamm, M. and Stolz, D. (2012) Aspergillus-PCR in bronchoalveolar lavage for detection of invasive pulmonary aspergillosis in immunocompromised patients. BMC Infectious Diseases 12: 237-244.

Specimen requirements: Throat or respiratory swab, or 0.2 ml water, or fungal culture, or 0.2 ml fresh, frozen or fixed tissue, or environmental surface swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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