Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Coronavirus PCR test

wildlife and zoo assay data sheet

Coronaviruses in various animal species

Test code:
S0140 - Ultrasensitive qualitative detection of various coronaviruses by reverse transcription coupled real time PCR. This assay detects but does not differentiate several coronaviruses, including those of equines, bovines, white-tailed deer, elk, sambar deer, waterbuck, giraffe and sable antelope.

Test S0140 is included in P0047 - ruminant fecal screening panel

Coronaviruses are RNA viruses that mutate quickly to adapt to different environments.  This significantly increases their potential for interspecies transmission. SARS (severe acute respiratory syndrome) is an example of zoonotic infection of humans by a group 2b coronavirus.

Coronaviruses have now been recognized as important pathogens in several captive or wild ruminant species in the United States (Alekseev et al., 2008), including white-tailed deer (Odocoileus virginianus), elk (Cervus elephus), sambar deer (Cervus unicolor) and waterbuck (Kobus ellipsiprymnus). In addition, some caribou (Rangifer tarandus), musk oxen (Ovibus moschatus) and sitatunga (Tragelaphus spekei) have been found to be seropositive for coronavirus.

Coronavirus infection can result in clinically mild-to-severe and sometimes bloody diarrhea. Similarly, coronaviruses have also been shown to cause diarrhea in many ruminants, including cattle, horses, deer and antelopes. In cattle, coronavirus infection can also cause respiratory symptoms.

Serological detection of coronaviruses has low sensitivity and is not very specific. However, PCR detection of coronaviruses can provide rapid, specific and sensitive determination of the presence of the virus.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of coronaviruses
  • Early prevention of spread of these viruses among a population
  • Minimize human exposure to the virus

References:
Alekseev, K.P., Vlasova, A.N., Jung, K., Hasoksuz, M., Zhang, X., Halpin, R., Wang, S., Ghedin, E., Spiro, D. and Saif, L.J. (2008) Bovine-like coronaviruses isolated from four species of captive wild ruminants are homologous to bovine coronaviruses, based on complete genomic sequences. J Virol. 82:12422-12431.

Specimen requirements:  0.2 ml feces, or fecal swab, or nasopharyngeal swab

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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